[1]余真,郑圆圆,黄族贵.孕期DEHP暴露后子代雌鼠下丘脑lncRNA的差异性表达研究[J].福建医药杂志,2023,45(06):123-127.
　YU Zhen,ZHENG Yuanyuan,HUANG Zugui.Differential expression of lncRNA in the hypothalamus of offspring female rats after DEHP uterus exposure[J].FUJIAN MEDICAL JOURNAL,2023,45(06):123-127.
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孕期DEHP暴露后子代雌鼠下丘脑lncRNA的差异性表达研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期数:
2023年06期
页码:
123-127
栏目:
基础研究
出版日期:
2023-12-15

文章信息/Info

Title:
Differential expression of lncRNA in the hypothalamus of offspring female rats after DEHP uterus exposure
文章编号:
1002-2600(2023)06-0123-05
作者:
余真; 郑圆圆1; 黄族贵2
福建省医学科学研究院 福建省医学测试重点实验室(福州 350001)
Author(s):
YU Zhen;  ZHENG Yuanyuan;  HUANG Zugui
Fujian Provincial Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou, Fujian 350001, China
关键词:
RNA-seq;  大鼠;  DEHP;  长链非编码RNA;  下丘脑
Keywords:
RNA-seq;  rats;  DEHP;  lncRNA;  hypothalamus
分类号:
R114
文献标志码:
A
摘要:
目的 探究孕期DEHP暴露对大鼠子代雌鼠下丘脑长链非编码RNA(lncRNA)表达的影响,分析并预测DEHP影响子代下丘脑-垂体-性腺轴与能量代谢的机制。方法 大鼠孕0～18 d连续灌胃染毒,子代雌鼠出生后立刻取下丘脑组织并提取总RNA,使用illumina Hiseq测序平台进行测序,筛选出差异表达的mRNA和lncRNA进行GO与KEGG富集分析。利用实时荧光定量PCR检测技术验证染毒组与对照组的下丘脑细胞中差异表达的目的lncRNA。结果 通过RNA-seq测序分析,筛查到108条差异表达的lncRNA和342条差异表达的mRNA。KEGG分析显示差异表达的lncRNA主要富集在雌激素信号通路、Ras信号通路信号转导和PI3K-Akt信号通路等通路。结论 孕期DEHP暴露后子代雌鼠下丘脑组织存在lncRNA及mRNA的差异表达,且差异表达的lncRNA和mRNA功能分析结果显示产前DEHP暴露可能对子代雌鼠下丘脑的性腺激素和能量代谢过程有影响。
Abstract:
Objective To investigate the effect of DEHP uterus exposure on the expression of lncRNA in the hypothalamus of offspring female rats, and to analyze and predict the mechanism by which DEHP affects the hypothalamic pituitary gonadal axis and energy metabolism in offspring.Methods Rats were exposed to DEHP continuously by gavage from 0 to 18 days since pregnant.The hypothalamic tissue of the offspring female rats was immediately taken after birth and total RNA was extracted.The Illumina Hiseq sequencing platform was used for sequencing, and differentially expressed mRNA and lncRNA were screened for GO and KEGG enrichment analysis.Results The results showed that 108 significantly differentially expressed lncRNA and 342 significantly differentially expressed mRNA were identified through RNA-seq sequencing analysis.KEGG analysis showed that differentially expressed lncRNA was mainly enriched in estrogen signaling pathway, Ras signaling pathway signal transduction, and PI3K-Akt signaling pathway.Conclusion There are differentially expressed lncRNA and mRNA in hypothalamic tissue of female offspring after DEHP exposure during pregnancy, and the functional analysis results of differentially expressed lncRNA and mRNA indicate that prenatal DEHP exposure may affect the gonadal hormone and energy metabolism process in hypothalamus of female offspring.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福建省卫生健康青年科研课题(2021QNB002); 福建省卫生计生中青年骨干人才培养项目(2018-ZQN-21); 福建省属公益类科研院所基本科研专项((2019R1011-2)
1 福建省立医院干部特诊二科; 2 福建医科大学附属南平第一医院,通信作者, Email:361331701@qq.com
更新日期/Last Update: 2023-12-15