[1]李业森.特异性靶向高转移骨肉瘤的长循环型PET探针的合成及显像[J].福建医药杂志,2023,45(06):127-129.
　LI Yesen.Synthesis and imaging of long-circulating PET probes specifically targeting highly metastatic osteosarcoma[J].FUJIAN MEDICAL JOURNAL,2023,45(06):127-129.
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特异性靶向高转移骨肉瘤的长循环型PET探针的合成及显像()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期数:
2023年06期
页码:
127-129
栏目:
基础研究
出版日期:
2023-12-15

文章信息/Info

Title:
Synthesis and imaging of long-circulating PET probes specifically targeting highly metastatic osteosarcoma
文章编号:
1002-2600(2023)06-0127-03
作者:
李业森
厦门大学附属第一医院核医学科(厦门 361003)
Author(s):
LI Yesen
Department of Nuclear Medicine, the First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, China
关键词:
PET;  骨肉瘤;  [64Cu]Cu-DOTA-EB-TMTP1;  64Cu标记
Keywords:
PET;  osteosarcoma;  [64Cu]Cu-DOTA-EB-TMTP1;  64Cu labelling
分类号:
R814
文献标志码:
B
摘要:
目的 研究[64Cu]Cu-DOTA-EB-TMTP1对143B骨肉瘤荷瘤小鼠模型的诊断效果。方法 多肽TMTP1(NVVRQ)对高转移性细胞有很好的靶向性,本研究利用64Cu标记DOTA-EB-TMTP1合成[64Cu]Cu-DOTA-EB-TMTP1,然后对143B骨肉瘤肝癌荷瘤小鼠进行microPET/CT显像,研究其对肿瘤的诊断效果。结果 [64Cu]Cu-DOTA-EB-TMTP1的放射性标记产率为(87.3±5.2)%,放化纯度大于95.0%。实验证明经过伊文思蓝(Evans Blue)修饰后的TMTP1,具有更高的肿瘤摄取和更长的血液半衰期,8 h肿瘤摄取值可达(6.50±0.88)% ID/g。结论 对于骨肉瘤的诊断来说,[64Cu]Cu-DOTA-EB-TMTP1可能是更好的PET探针,更有利于肿瘤的诊疗一体化转化应用。
Abstract:
Objective To assess the diagnostic effectiveness of [64Cu]Cu-DOTA-EB-TMTP1 in a mouse model of 143B osteosarcoma.Methods Polypeptide TMTP1(NVVRQ)had good targeting effect on highly metastatic cells.[64Cu]Cu-DOTA-EB-TMTP1 was synthesized and subsequently used for microPET/CT imaging in a mouse model of 143B osteosarcoma to investigate its diagnostic performance.Results The radiolabeling yield of [64Cu]Cu-DOTA-EB-TMTP1 was(87.3±5.2)%, and the radiochemical purity exceeded 95.0%.The results demonstrated that TMTP1, modified with Evans Blue, exhibited increased tumor uptake and a longer blood half-life.Tumor uptake values reached(6.50±0.88)% ID/g at eight hours.Conclusion [64Cu]Cu-DOTA-EB-TMTP1 may represent a superior PET probe for the diagnosis of osteosarcoma, facilitating the integration of diagnosis and therapy for this condition.

参考文献/References:

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[5] 李业森,张德良,张现忠,等.18F-AlF-NOTA-G-TMTP1的合成及对高转移潜能肝癌细胞荷瘤鼠的显像研究[J].中华核医学与分子影像杂志,2015,35(5):351-354.
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更新日期/Last Update: 2023-12-15