[1]程变巧 林园香 黄志腾 霍苗苗.IL-1诱导肝癌小鼠炎症及免疫反应的实验研究[J].福建医药杂志,2020,42(06):126-128.
　CHENG Bianqiao,LIN Yuanxiang,HUANG Zhiteng,et al.Experimental study on inflammation and immune response induced by IL-1 in liver cancer mice[J].FUJIAN MEDICAL JOURNAL,2020,42(06):126-128.
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IL-1诱导肝癌小鼠炎症及免疫反应的实验研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期数:
2020年06期
页码:
126-128
栏目:
基础研究
出版日期:
2020-12-15

文章信息/Info

Title:
Experimental study on inflammation and immune response induced by IL-1 in liver cancer mice
文章编号:
1002-2600(2020)06-0126-03
作者:
程变巧 林园香 黄志腾 霍苗苗
厦门大学附属福州第二医院消化内科(福州 350007)
Author(s):
CHENG Bianqiao; LIN Yuanxiang; HUANG Zhiteng; HUO Miaomiao.
Department of Gastroenterology,Fuzhou Second Hospital Affiliated to Xxiamen University, Fuzhou,Fujian 350007,China
关键词:
肝癌微环境;  炎症;  免疫;  肝癌模型
Keywords:
hepatocarcinoma microenvironment;  inflammation;  immunity;  hepatocarcinoma model
分类号:
R735.7 R392
文献标志码:
B
摘要:
目的 通过建立肝癌小鼠模型,观察IL-1刺激肝癌小鼠后对肝癌炎症及免疫微环境的影响。方法 饲养20只NPG级小鼠,予SMMC-7721肝癌细胞在肝包膜下直接接种,待瘤体长出后,随机分为IL-1干预组和对照组,ELISA方法观察IL-10和IL-8干预前后的表达,流式细胞术观察CD4+和CD25+的表达情况。结果 小鼠有17只成瘤,成瘤率为85%。把17只成瘤小鼠分为对照组7只,干预组10只; 干预组予IL-1腹腔连续注射3 d后发现有6只瘤鼠死亡,4只精神萎靡,5 d后小鼠全部死亡; 对照组小鼠无死亡情况。IL-8在干预后的表达较干预前明显上调(P<0.05),IL-10 在干预后的表达较干预前升高,但两组比较,差异无统计学意义(P>0.05)。CD4+CD25+占CD4+的比值在干预后的表达均较干预前有上调(P<0.05)。结论 肝癌小鼠内存在炎症和免疫反应,IL-1可以诱导肝癌小鼠的炎症和免疫反应强化。
Abstract:
Objective To establish a mouse model of liver cancer to observe the effects on the inflammation and immune microenvironment of liver cancer in mice stimulated by IL-1.Methods Atotal of twenty NPG mice were bred and inoculated directly under the liver capsule with smmc-7721 hepatocarcinoma cell line.After tumor growth, they were randomly divided into the IL-1intervention group and the non-intervention group.The expressions of IL-10 and IL-8 before and after intervention were observed by ELISA, and the expressions of CD4+ and CD4+CD25+ were observed by flow cytometry.Results Tumorigenesis rate was 85% in 20 NPG mice.The 17 tumor-forming nude mice were divided into control group(7)and intervention group(10).After 3 days of continuous intraperitoneal injection of IL-1, 6 mice in the intervention group died and 4 mice were found to be listless.All the mice died on the fifth day.The control group had no death.Expression of IL-8,IL-10 and CD4+CD25+ were detected in the control group.IL-8 after the intervention of expression significantly raised(P<0.05), compared with before, IL-10 after the intervention in the expression of a higher before and after the intervention, but no obvious difference compared the two groups(P>0.05).The expression of CD4+CD25+/CD4+ after intervention have been raised(P<0.05), compared with before.Expression of IL-8,IL-10 and CD4+CD25+ were detected in the control group.Conclusion There are inflammatory and immune responses in liver cancer mice, and IL-1 can induce inflammation and immune enhancement in liver cancer mice.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福州市卫生计生科技计划项目(2017-S-wt1)
更新日期/Last Update: 1900-01-01