[1]郑飞,李建成.长链非编码RNA MALAT1对肺癌细胞放疗敏感性的作用[J].福建医药杂志,2021,43(06):125-128.
　ZHENG Fei,LI Jiancheng.Effect of long non-coding RNA MALAT1 on radiosensitivity of lung cancer cells[J].FUJIAN MEDICAL JOURNAL,2021,43(06):125-128.
点击复制

长链非编码RNA MALAT1对肺癌细胞放疗敏感性的作用()
分享到：

《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
43
期数:
2021年06期
页码:
125-128
栏目:
基础研究
出版日期:
2021-12-15

文章信息/Info

Title:
Effect of long non-coding RNA MALAT1 on radiosensitivity of lung cancer cells
文章编号:
1002-2600(2021)06-0125-04
作者:
郑飞; 李建成1
福建省肿瘤医院 福建医科大学附属肿瘤医院放疗科(福州 350014)
Author(s):
ZHENG Fei;  LI Jiancheng
Department of Radiology,Fujian Cancer Hospital,Fujian Medical University Cancer Hospital,Fuzhou, Fujian 350014,China
关键词:
肺癌;  放射治疗;  长链非编码RNA
Keywords:
lung cancer;  radiotherapy;  long non-coding RNA
分类号:
R734.2
文献标志码:
A
摘要:
目的 研究长链非编码RNA(LncRNA)肺腺癌转移相关转录本1(MALAT1)对肺癌细胞放射治疗敏感性的作用。方法 运用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测肺癌细胞A549中MALAT1的表达,应用慢病毒在A549细胞中干扰或者过表达MALAT1,并通过克隆形成实验检测干扰或者过表达MALAT1后A549细胞放疗敏感性的改变,通过Western blot检测干扰或者过表达MALAT1后A549细胞放疗后DNA损伤标记物γ-h2ax表达的改变。结果 通过慢病毒在A549细胞中干扰MALAT1后,A549细胞的放射敏感性增加,放疗后DNA损伤标记物γ-h2ax的表达增加。通过慢病毒在A549细胞中过表达MALAT1后,A549细胞的放射敏感性降低,放疗后DNA损伤标记物γ-h2ax的表达减少。结论 调控长链非编码RNA MALAT1可以影响肺癌细胞的放射治疗敏感性,且有可能成为肺癌放疗增敏的新靶点。
Abstract:
Objective To study the effect of long non-coding RNA MALAT1 on radiosensitivity of lung cancer cells.Methods qRT-PCR was used to detect the expression of MALAT1 in lung cancer cells A549, lentivirus was used to knockdown or overexpress MALAT1 in A549 cells, and clone formation experiment was used to detect the changes in radiosensitivity of A549 cells after knockdown or overexpression of MALAT1, Western blot was used to detect the changes in the expression of γ-h2ax, a radiation induced DNA damage marker in A549 cells after knockdown or overexpression of MALAT1.Results After the knockdown of MALAT1 in A549 cells by lentivirus, the radiosensitivity of A549 cells increased, and the expression of DNA damage marker γ-h2ax increased after radiotherapy.After overexpressing MALAT1 in A549 cells by lentivirus, the radiosensitivity of A549 cells was reduced, and the expression of DNA damage marker γ-h2ax after radiotherapy was reduced.Conclusion The regulation of long non-coding RNA MALAT1 can affect the radiosensitivity of lung cancer cells, and it may become a new target for lung cancer radiotherapy sensitization.

参考文献/References:

[1] 郑荣寿,孙可欣,张思维,等.2015年中国恶性肿瘤流行情况分析[J].中华肿瘤杂志,2019,41(1):19-28.
[2] Brown S, Banfill K, Aznar M C, et al.The evolving role of radiotherapy in non-small cell lung cancer[J].Br J Radiol,2019,92(1104):20190524.
[3] Baker S, Dahele M, Lagerwaard F J, et al.A critical review of recent developments in radiotherapy for non-small cell lung cancer[J].Radiat Oncol,2016,11(1):115.
[4] Jiang M C, Ni J J, Cui W Y, et al.Emerging roles of lncRNA in cancer and therapeutic opportunities[J].Am J Cancer Res,2019,9(7):1354-1366.
[5] Li Z X, Zhu Q N, Zhang H B, et al.MALAT1: a potential biomarker in cancer[J].Cancer Manag Res,2018,10:6757-6768.
[6] Ma J, Wu K, Liu K, et al.Effects of MALAT1 on proliferation and apo- ptosis of human non-small cell lung cancer A549 cells in vitro and tumor xenograft growth in vivo by modulating autophagy[J].Cancer Biomark,2018,22(1):63-72.
[7] 中华医学会放射肿瘤治疗学分会,中国医师协会放射肿瘤治疗医师分会,中国抗癌协会放射治疗专业委员会,等.中国非小细胞肺癌放射治疗临床指南(2020版)[J].中华放射肿瘤学杂志,2020,29(8):599-607.
[8] Wu J, Weng Y, He F, et al.LncRNA MALAT-1 compe-titively regulates miR-124 to promote EMT and development of non-small-cell lung cancer[J].Anticancer Drugs,2018,29(7):628-636.
[9] 孙秀凤,周芬,吴婷.长链非编码RNA MALAT1对NSCLS增殖、侵袭迁移及凋亡的影响[J].中国肿瘤外科杂志,2020,12(2):148-152,157.
[10] Yang T, Li H, Chen T, et al.LncRNA MALAT1 depressed chemo-sensitivity of NSCLC cells through directly functioning on miR-197-3p/p120 catenin axis[J].Mol Cells,2019,42(3):270-283.
[11] Huang Q.Predictive relevance of ncRNAs in non-small-cell lung cancer patients with radiotherapy: a review of the published data[J].Biomark Med,2018,12(10):1149-1159.

相似文献/References:

[1]黄郴,徐驯宇.胸腔镜外科领域新进展[J].福建医药杂志,2017,39(4):1.
[2]谢宝松.原发性肺癌规范化诊断——谢宝松教授解读CSCO肺癌指南2018版(视频+典型病例)[J].福建医药杂志,2019,41(06):163.
[3]王欣,林明星,陈全文,等.VISTA和PD-1信号通路联合阻断在小鼠结肠癌放射治疗中的作用机制[J].福建医药杂志,2022,44(01):120.
[4]陈新旺,薛聃,黄峥慧,等.慢性阻塞性肺疾病对肺癌患者免疫治疗相关性肺炎风险影响的Meta分析[J].福建医药杂志,2022,44(06):25.
　CHEN Xinwang,XUE Dan,HUANG Zhenghui,et al.Impact of chronic obstructive pulmonary disease on risk of immune checkpoint inhibitor pneumonitis in patients with lung cancer: a meta-analysis[J].FUJIAN MEDICAL JOURNAL,2022,44(06):25.
[5]李蓉,宋继红,郑雪冰,等.鼻咽癌患者放疗期间音乐体适能活动方案的构建研究[J].福建医药杂志,2023,45(03):142.

备注/Memo

备注/Memo:
1 通信作者,Email: jianchengli6@126.com
更新日期/Last Update: 2021-12-15