[1]陈 珊 陈文旭 徐 迪 李立帜.肥厚性幽门狭窄大鼠幽门中神经生长因子及其受体TrkA、p75NTR的表达[J].福建医药杂志,2020,42(04):134-136.
　CHEN Shan,CHEN Wenxu,XU Di,et al.Expression of nerve growth factor and its receptors TrkA and p75NTR in mice with hypertrophic pyloric stricture[J].FUJIAN MEDICAL JOURNAL,2020,42(04):134-136.
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肥厚性幽门狭窄大鼠幽门中神经生长因子及其受体TrkA、p75NTR的表达()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期数:
2020年04期
页码:
134-136
栏目:
基础研究
出版日期:
2020-08-20

文章信息/Info

Title:
Expression of nerve growth factor and its receptors TrkA and p75NTR in mice with hypertrophic pyloric stricture
文章编号:
1002-2600(2020)04-0134-03
作者:
陈 珊 陈文旭 徐 迪1 李立帜1
厦门大学附属福州第二医院检验科(福州 350013)
Author(s):
CHEN Shan;  CHEN Wenxu;  XU Di;  LI Lizhi.
Department of Clinical Laboratory, Fuzhou Second Hospital Affiliated to Xiamen University, Fuzhou, Fujian 350013, China
关键词:
先天性肥厚性幽门狭窄;  神经生长因子;  TrkA;  p75NTR
Keywords:
congenital hypertrophic pyloric stenosis;  nerve growth factor;  TrkA;  p75NTR
分类号:
R573
文献标志码:
A
摘要:
目的 研究肥厚性幽门狭窄大鼠幽门中神经生长因子(NGF)及神经营养因子酪氨酸激酶受体A(TrkA)、p75神经营养因子受体(p75NTR)表达情况,探讨NGF及其受体TrkA与p75NTR在先天性肥厚性幽门狭窄起病中的作用。方法 将受孕后的SD大鼠随机分为模型组与对照组,模型组经尾静脉注射给予N-硝基-左旋精氨酸甲酯(L-NAME)20 mg/(kg·d),对照组经尾静脉注射给予同等剂量的生理盐水。新生鼠于出生后第1、7、14天与第21天分别称重,生后第2天麻醉处死,留取幽门组织。HE染色后显微镜下测量幽门肌层厚度。实时定量聚合酶链反应(RT-PCR)检测幽门组织中的NGF及其受体TrkA与p75NTR的mRNA表达水平。Western blot检测幽门组织中的NGF及其受体TrkA与p75NT的蛋白质表达水平。结果 与对照组新生鼠比较,模型组新生鼠体质量增长明显缓慢,体质量较低。模型组幽门肌明显肥厚。模型组新生鼠NGF的mRNA及蛋白表达显著减少; 模型组新生鼠TrkA与p75NTR的mRNA及蛋白表达显著减低,差异有统计学意义(P<0.05)。结论 幽门组织中TrkA与p75NTR降低及NGF的表达减少可能与先天性肥厚性幽门狭窄的发病有关。
Abstract:
Objective To study the expression of nerve growth factor(NGF), neurotrophic factor tyrosine kinase receptor A(TrkA)and p75 neurotrophic factor receptor(p75NTR)in the pylorus of mice with hypertrophic pyloric stenosis, and to explore the role of NGF and its receptors TrkA and p75NTR in the onset of congenital hypertrophic portal stenosis. Methods Pregnant SD mice were randomly divided into model group and control group. The model group was given 20 mg/(kg·d)of N-nitro-L-arginine methyl ester(L-NAME)by tail vein injection, while the control group was given the same dose of normal saline through tail vein. The newborn mice were weighed on the 1st, 7th, 14th and 21st day after birth. The mice were killed on the 2nd day after birth. The thickness of pylorus muscle layer was measured under microscope after staining. Real time quantitative polymerase chain reaction(RT-PCR)was used to detect the mRNA expression levels of NGF, TrkA and p75NTR in pyloric tissue. Western blot was used to detect the protein expression of NGF, TrkA and p75NTR. Results Compared with the control group, the body weight of the model group was significantly slower than that of the control group. In the model group, the pyloric muscle was obviously hypertrophic. The expression of NGF mRNA and protein was significantly decreased in the model group, and the mRNA and protein expression of TrkA and p75NTR in the model group were significantly decreased(P<0.05). Conclusion The decrease of TrkA, p75NTR and NGF expression in pyloric tissue may be related to the pathogenesis of CHPS.

参考文献/References:

[1] 蔡本龙,张又祥.先天性肥厚性幽门狭窄手术方式研究进展[J].国际儿科学杂志,2016,43(3):201-203.
[2] 李万福,马柱,李朝旺,等.腹腔镜幽门环肌切开术与开腹手术治疗先天性肥厚性幽门狭窄有效性和安全性Meta分析[J].中华实用儿科临床杂志,2013,28(18):1433-1436.
[3] 曲颜,刘远梅.胶质细胞源性神经营养因子在肠神经系统中的作用[J].中华实用儿科临床杂志,2014,29(5):384-386.
[4] 高颜,王春晖,林燕,等.红霉素对胃食管反流幼兔模型胃肠道动力作用的影响[J].中华实用儿科临床杂志,2016,31(07):491-495.
[5] Gezer H ?Oguzkurt P,Temiz A,et al.Hypertrophic pyloric stenosis in twins; genetic or environmental factors[J].Clin Genet,2015,87(4):388-391.
[6] Boybeyi O,Soyer T,A tasoy P,et al.Investigation of the effects of charm Horm ones Oil the pyloric muscle in newborn rats[J].J Pediatr Surg,2015,50(3):408-412.
[7] 魏颖,张又祥,张榕培,等.肝细胞生长因子在先天性肥厚性幽门狭窄大鼠幽门中的表达[J].广东医学,2015,36(12):1855-1858.

备注/Memo

备注/Memo:
1 福建医科大学省立临床医学院 福建省立医院小儿外科
更新日期/Last Update: 2020-08-20