[1]杨国华,郑圣斌,游治杰,等.FGF19因子促进结肠癌细胞增殖及迁移的机制研究[J].福建医药杂志,2024,46(05):56-59.[doi:10.20148/j.fmj.2024.05.016]
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FGF19因子促进结肠癌细胞增殖及迁移的机制研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年05期
页码:
56-59
栏目:
基础研究
出版日期:
2024-10-15

文章信息/Info

文章编号:
1002-2600(2024)05-0056-04
作者:
杨国华郑圣斌游治杰林娟
福建医科大学省立临床医学院 福建省立医院南院,福州 350007
关键词:
结肠癌 FGF19 增殖 迁移
分类号:
R735.3
DOI:
10.20148/j.fmj.2024.05.016
文献标志码:
B
摘要:
目的 分析FGF19在结肠癌中的表达、对预后的影响及可能的机制。方法 通过UALCAN平台分析FGF19在结肠癌中的表达情况; 通过Kaplan-Meier plotter平台分析FGF19对结肠癌生存时间(OS)及无复发生存时间(RFS)的影响。细胞实验分3组:对照组、FGF19组、FGF19+FGF401组,CCK8法比较第5天时细胞A值和划痕实验观察24 h后迁移率,Western blot观察3组细胞内AKT、ERK、STAT3、pAKT、pERK、pSTAT3相对表达量。结果 FGF19在结肠癌中的表达明显高于正常结肠组织,差异有统计学意义(P<0.05); FGF19降低OS及RFS,与低表达组比较,差异有统计学意义(P<0.05)。第5天时FGF19组细胞A值(2.76±0.18)、对照组A值(1.36±0.04)、FGF19+FGF401组A值(1.70±0.03),3组比较,差异有统计学意义(F=101.5,P<0.01); 而FGF19组24 h迁移率(0.56±0.04)、对照组(0.38±0.14)、FGF19+FGF401组迁移率(0.40±0.05),3组比较,差异无统计学意义(F=2.699,P>0.05); FGF19组pAKT(0.24±0.03)、对照组(0.20±0.02)与FGF19抑制组pAKT(0.07±0.10)比较,差异有统计学意义(F=5.858,P<0.05); FGF19组pSTAT3(0.08±0.006)、对照组(0.09±0.02)、FGF19+FGF401组pSTAT3(0.003±0.001),3组比较,差异有统计学意义(F=66.84,P<0.01)。结论 FGF19可能通过AKT、STAT3蛋白磷酸化,促进结肠癌生长及迁移,降低结肠癌患者的OS及PFS。

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备注/Memo

备注/Memo:
基金项目:福建省卫健康委中青年骨干人才培养项目(2020GGB007)
更新日期/Last Update: 2024-10-15