[1]翁一玲,苏艺萍,李煌,等.畅脉乐胶囊对MCAO大刀产脑组织保护作用研究及mGluR1、GABAB蛋白表达的影响[J].福建医药杂志,2022,44(05):124-127.
　WENG Yiling,SU Yiping,Li Huang,et al.Study on the protective effect of Changmaile Capsule on MCAO rat brain tissue and the expression of mGluR1 and GABAB[J].FUJIAN MEDICAL JOURNAL,2022,44(05):124-127.
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畅脉乐胶囊对MCAO大刀产脑组织保护作用研究及mGluR1、GABAB蛋白表达的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期数:
2022年05期
页码:
124-127
栏目:
基础研究
出版日期:
2022-10-15

文章信息/Info

Title:
Study on the protective effect of Changmaile Capsule on MCAO rat brain tissue and the expression of mGluR1 and GABAB
作者:
翁一玲; 苏艺萍1; 李煌1; 陈松旺2
福建中医药大学附属第二人民医院药学部（福州 350001）
Author(s):
WENG Yiling; SU Yiping; Li Huang; CHEN Songwang
The second Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fuzhou, Fujian 350001, China
关键词:
畅脉乐胶囊; 脑缺血; mGluR1; GABAB
Keywords:
Changmaile capsule; Cerebral ischemia; mGluR1; GABAB
分类号:
R743.3
文献标志码:
A
摘要:
目的 探讨畅脉乐胶囊对脑缺血再灌注损伤（MCAO）大鼠脑组织的保护机制。 方法 采用线栓法制备MCAO大鼠模型，随机分为3组，畅脉乐组采用灌胃方式给药，每日1次，连续给药7天；假手术组和模型组给予等剂量的生理盐水。采用mNSS法进行神经功能缺陷评分；TTC染色计算梗死面积和HE染色观察脑损伤情况；采用 Real-Time PCR 法和 Western blot 法检测mGluR1和GABAB mRNA 蛋白表达情况。 结果 与模型组相比，畅脉乐组神经功能缺损程度评分显著降低，梗死面积显著减少（P<0.05）。畅脉乐组mGluR1 mRNA和蛋白表达水平明显降低，GABAB mRNA 和蛋白表达水平明显升高（P<0.05）。 结论 畅脉乐胶囊可治疗大鼠脑缺血损伤并调节脑组织mGluR1和GABAB蛋白水平。
Abstract:
Objective To investigate the protective mechanism of Changmai Le capsule on MCAO rat brain tissue. Method The Middle cerebral artery occlusion (MCAO) rat model was established by suture occlusion method. The rats were randomly divided into 3 groups. After the modeling was successful, Changmaile group was given CMLC contents by intragastric administration, once a day, for consecutive 7 days. Sham operation group and model group were given equal volume of normal saline. MNss method was used to score neurological deficits. TTC staining was used to calculate infarct volume and HE staining was used to observe brain injury. The mRNA and protein expressions of mGluR1 and GABAB were detected by real-time PCR and Western blot. Results Compared with model group, the score and infarct volume of Changmai Le group were significantly decreased (P<0.05). In addition, the mRNA and protein expression levels of mGluR1 in Changmai Group were significantly decreased, while the mRNA and protein expression levels of GABAB were significantly increased (P<0.05). Conclusion Changmai Le capsule can treat cerebral ischemia injury and regulate the levels of mGluR1 and GABAB protein in the brain of rats.

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相似文献/References:

[1]陈松旺 吴 婷 李 煌 翁一玲.畅脉乐胶囊的质量标准研究[J].福建医药杂志,2020,42(01):127.
　CHEN Songwang,WU Ting,LI Huang,et al.Quality standard for changmaile capsules[J].FUJIAN MEDICAL JOURNAL,2020,42(05):127.

备注/Memo

备注/Memo:
基金项目：福建省自然科学基金面上项目（2019J01482）
1福建中医药大学药学院；2福建省药品审核查验中心；3通讯作者，Email：724405204@qq.com
更新日期/Last Update: 2022-10-15