[1]李业森.68Ga标记TMTP1探针在肝癌肿瘤模型中的microPET/CT显像研究[J].福建医药杂志,2022,44(05):122-124.
　Li Yesen.Study on microPET/CT imaging of 68GA-labeled TMTP1 probe in HCC tumor model[J].FUJIAN MEDICAL JOURNAL,2022,44(05):122-124.
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68Ga标记TMTP1探针在肝癌肿瘤模型中的microPET/CT显像研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期数:
2022年05期
页码:
122-124
栏目:
基础研究
出版日期:
2022-10-15

文章信息/Info

Title:
Study on microPET/CT imaging of 68GA-labeled TMTP1 probe in HCC tumor model
作者:
李业森
厦门大学附属第一医院核医学科（厦门 361003）
Author(s):
Li Yesen
Department of Nuclear Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian361003, China
关键词:
PET; 肝癌; 68Ga[Ga]-NOTA-PEG2-TMTP1; 68Ga标记
分类号:
R735.7
文献标志码:
A
摘要:
目的 研究68Ga[Ga]-NOTA-PEG2-TMTP1对SMMC-7721肝癌荷瘤小鼠模型的诊断效果。 方法 氨肽酶P2在一些肿瘤(如肝癌、肺癌、乳腺癌等)中有高表达。TMTP1可以特异性地靶向氨肽酶P2，本研究利用68Ga标记NOTA-PEG2-TMTP1合成68Ga[Ga]-NOTA-PEG2-TMTP1，然后对SMMC-7721肝癌荷瘤小鼠进行microPET/CT显像研究，研究其对肿瘤的诊断效果。 结果 68Ga[Ga]-NOTA-PEG2-TMTP1的放射性标记产率为87.16±1.23%，放化纯度大于99.0%。实验证明相PEG修饰后的TMTP1，具有更好的肿瘤靶向性，肿瘤肌肉比可以高达5.66。 结论 对于肝癌的诊断来说，68Ga[Ga]-NOTA-PEG2-TMTP1可能是更好的PET探针，具有进一步临床转化的前景。
Abstract:
Objective To investigate the diagnostic effect of 68Ga[Ga]-NOTA-PEG2-TMTP1 on a mouse model of SMMC-7721 hepatocellular carcinoma in nude mice. Methods Aminopeptidase P2 was highly expressed in some tumors (e.g., liver, lung, breast cancer, etc.). TMTP1 could specifically target aminopeptidase P2. In this study, 68Ga[Ga]-NOTA-PEG2-TMTP1 was synthesized using 68Ga-labeled NOTA-PEG2-TMTP1, then SMMC-7721 hepatocellular carcinoma tumor-bearing nude mice for microPET/CT imaging study to investigate its diagnostic effect on tumors. Results The radiolabeling yield of 68Ga[Ga]-NOTA-PEG2-TMTP1 was 87.16±1.23%, and the radiochemical purity was greater than 99.0%.It was demonstrated that TMTP1, after phase PEG modification, had better tumor targeting, with tumor muscle ratio up to 5.66.Conclusion For the diagnosis of hepatocellular carcinoma, 68Ga[Ga]-NOTA-PEG2-TMTP1 may be a better PET probe with the prospect of further clinical translation.

参考文献/References:

[1] Diamantis I T, Fabio B, Kota S, et al.Prognosis After Resection of Barcelona Clinic Liver Cancer (BCLC) Stage 0, A, and B Hepatocellular Carcinoma: A Comprehensive Assessment of the Current BCLC Classification [J].Ann Surg Oncol, 2019 26(11):3693-3700.
[2] Marlène P, Régis V, Céline F, et al.Stability of peptides and therapeutic success in cancer [J].Expert Opin Drug Metab Toxicol, 2011, 7(7):793-802.
[3] 陈小元, 18F-阿法肽的基础研发及临床转化历程 [J].中华核医学与分子影像杂志, 2019, 39 (4)： 193-195.
[4] Fei L, Teng C, Qingjian D, et al.Evaluation of (99m)Tc-HYNIC-TMTP1 as a tumor-homing imaging agent targeting metastasis with SPECT [J].Nucl Med Biol, 2015, 42(3):256-262.
[5] Yesen L, Deliang Z，Ying S, et al.Syntheses and preliminary evaluation of [(18) F]AlF-NOTA-G-TMTP1 for PET imaging of high aggressive hepatocellular carcinoma [J].Contrast Media Mol Imaging, 2016, 11(4):262-271.
[6] 李业森, 张德良, 张现忠等, 18F-AlF-NOTA-G-TMTP1的合成及对高转移潜能肝癌细胞荷瘤鼠的显像研究[J].中华核医学与分子影像杂志, 2015, 35 (5): 351-354.

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备注/Memo

备注/Memo:
基金项目：福建省自然科学基金资助项目(2020J011224)
更新日期/Last Update: 2022-10-15