[1]陈冬冬,陈嵘.microRNA-365对骨肉瘤大鼠巨噬细胞增殖和凋亡的影响[J].福建医药杂志,2022,44(02):116-119.
　CHEN Dongdong,CHEN Rong.Effect of microRNA-365 on macrophage proliferation and apoptosis in osteosarcoma rats[J].FUJIAN MEDICAL JOURNAL,2022,44(02):116-119.
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microRNA-365对骨肉瘤大鼠巨噬细胞增殖和凋亡的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期数:
2022年02期
页码:
116-119
栏目:
基础研究
出版日期:
2022-04-15

文章信息/Info

Title:
Effect of microRNA-365 on macrophage proliferation and apoptosis in osteosarcoma rats
文章编号:
1002-2600(2022)02-0116-04
作者:
陈冬冬; 陈嵘1
厦门大学附属福州第二医院骨肿瘤科(福州 350007)
Author(s):
CHEN Dongdong;  CHEN Rong
Department of Orthopaedics Oncology, the Affiliated Fuzhou Municipal Second Hospital, Xiamen University, Fuzhou, Fujian 350007, China
关键词:
miR-365;  骨肉瘤;  巨噬细胞
Keywords:
miR-365;  osteosarcoma;  macrophage
分类号:
R738.1
文献标志码:
B
摘要:
目的 探讨microRNA-365(miR-365)对骨肉瘤大鼠巨噬细胞增殖和凋亡的影响。方法 构建骨肉瘤大鼠模型,采用右后肢外侧皮下注射的方法将miR-365模拟物、miR-365阴性对照物、miR-365 抑制物、miR-365 抑制阴性对照物及生理盐水注入到大鼠模型中,采用RT-PCR检测骨肉瘤大鼠巨噬细胞miR-365、KRAS的mRNA表达,Western blot检测骨肉瘤大鼠巨噬细胞周期相关蛋白cyclin D1 和 cyclin E的表达。结果 与miR-365阴性对照组、miR-365抑制组、miR-365抑制阴性对照组、空白组相比较,miR-365组的miR-365、KRAS的mRNA表达量及cyclin D1、cyclin E的蛋白表达升高,差异性有统计学意义(P<0.05); 与miR-365抑制阴性对照组、空白组相比较,miR-365抑制组的miR-365、KRAS的mRNA表达量及cyclin D1、cyclin E的蛋白表达降低,差异有统计学意义(P<0.05); miR-365阴性对照组、miR-365抑制阴性对照组、空白组之间miR-365、KRAS的mRNA表达量及cyclin D1、cyclin E的蛋白表达差异无统计学意义(P>0.05)。结论 miR-365能够通过KRAS促进巨噬细胞的增殖,减轻生物体炎症反应。
Abstract:
Objective To investigate the effects of microRNA-365 on macrophage proliferation and apoptosis in osteosarcoma rats.Methods The osteosarcoma rats model was established.miR-365 simulant,miR-365 negative control,miR-365 inhibitor,miR-365 inhibitor negative control and normal saline were injected into the rat model.The mRNA expression of miR-365 and KRAS in macrophages of osteosarcoma rats was detected by RT-PCR, and the expression of cyclin D1 and cyclin E were detected by Western blot.Results Compared with miR-365 negative control group, miR-365 inhibition group, miR-365 inhibition negative control group and blank group, the mRNA expression levels of miR-365 and KRAS and the protein expression levels of cyclin D1 and cyclin E in miR-365 group were increased, and the differences were statistically significant(P<0.05).Compared with miR-365 inhibition negative control group and blank group, the mRNA expression of miR-365and KRAS and the protein expression of cyclin D1 and cyclin E in miR-365 inhibition group weredecreased,and the differences were statistically significant(P<0.05).There were no significant differences in the mRNA expression of miR-365 and KRAS and the protein expression of cyclin D1 and cyclin E between miR-365 negative control group, miR-365 inhibition negative control group and blank group(P>0.05).Conclusion miR-365 can promote the proliferation of macrophages through KRAS and reduce the inflammatory reaction of organism.

参考文献/References:

[1] 张清,徐万鹏,郭卫,等.我国骨肉瘤治疗现状及改进建议—17家骨肿瘤治疗中心1998～2008年资料分析[J].中国骨肿瘤骨病,2009,8(3):129-132.
[2] Kushlinskii N E,Fridman M V,Braga E A.Molecular mechanisms and microRNAs in osteosarcoma pathogenesis[J].Biochemistry(Mosc),2016,81(4):315-328.
[3] 陈冬冬,林佳生,严伟,等.胫骨内移植UMR-106成瘤组织块构建裸鼠骨肉瘤模型[J].福建医药杂志,2018,40(6):134-136,141.
[4] Sun R F,Liu Z G,Ma G,et al.Associations of deregulation of mir-365 and its target mRNA TTF-1 and survival in patients with NSCLC[J].Int J Clin Exp Pathol,2015,8(3):2392-2399.
[5] Chen Z Z,Huang Z F,Ye Q F,et al.Prognostic significance and antiproliferation effect of microRNA-365 in hepatocellular carcinoma[J].Int J Clin Exp Pathol,2015,8(2):1705-1711.
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备注/Memo

备注/Memo:
基金项目:2020年福州市卫生健康科研创新团队培育项目(2020-S-wt3)
1 通信作者,Email:chenrong_fjfz@126.com
更新日期/Last Update: 2022-04-15