[1]孙玉莹,黄春雨.NLRP3在小鼠氧诱导视网膜新生血管中的作用[J].福建医药杂志,2021,43(03):108-111.
　SUN Yuying,HUANG Chunyu.Role of NLRP3 in oxygen-induced retinal neovascularization in mice[J].FUJIAN MEDICAL JOURNAL,2021,43(03):108-111.
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NLRP3在小鼠氧诱导视网膜新生血管中的作用()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
43
期数:
2021年03期
页码:
108-111
栏目:
基础研究
出版日期:
2021-06-15

文章信息/Info

Title:
Role of NLRP3 in oxygen-induced retinal neovascularization in mice
文章编号:
1002-2600(2021)03-0108-05
作者:
孙玉莹; 黄春雨1
中山大学肿瘤防治中心防癌体检健康管理中心 华南肿瘤学国家重点实验室(广州 510060)
Author(s):
SUN Yuying;  HUANG Chunyu
Department of Cancer Prevention Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in SouthChina, Guangzhou, Guangdong 510060, China
关键词:
NLRP3:视网膜新生血管;  OIR动物模型;  NLRP3基因敲除小鼠
Keywords:
NLRP3;  retinal neovascularization;  OIR animal model;  NLRP3 gene knockout mice
分类号:
R-332 R774.1
文献标志码:
A
摘要:
目的 本研究旨在于小鼠氧诱导视网膜新生血管病变模型中探讨NLRP3-VEGF信号通路在视网膜新生血管形成中的作用。方法 用NLRP3基因敲除(NLRP3-/-)小鼠及对照的同种系C57BL/6J 小鼠建立氧诱导视网膜新生血管病变模型(OIR)。分别在P12、P17、P21天时取材,通过视网膜荧光灌注铺片,观察新生血管情况。选取建立OIR模型的正常C57BL/6J小鼠在P12、P17、P21天时取材,进行视网膜荧光灌注铺片,与OIR模型小鼠进行对比分析,验证OIR模型建立成功。两组OIR小鼠P17时取材,通过RT-PCR检测VEGF的RNA水平变化,评价NLRP3缺失对VEGF表达的调控作用。结果 视网膜荧光灌注铺片发现,在正常C57BL/6J未建立OIR模型时,视网膜未出现无灌注区和新生血管,说明OIR模型建立成功。在P12、P17和P21,与C57BL/6J的 OIR小鼠相比,在NLRP3-/- OIR小鼠中,新生血管的范围明显缩小,无灌注区减少,正常血管相对增多。P17时,与正常OIR小鼠相比,NLRP3-/- OIR的VEGF 表达水平显著下降。结论 通过对OIR小鼠视网膜新生血管病变进行研究,发现NLRP3参与调控VEGF的表达,影响视网膜新生血管的形成。本研究提出一条新的调控视网膜新生血管形成的通路,为视网膜血管损伤及新生血管形成的病理机制研究和治疗提供了新的思路和靶点。
Abstract:
Objective To explore the role of NLRP3-VEGF signal pathway in retinal neovascularization in mice with oxygen-induced retinal neovascularization. Methods Oxygen-induced retinal neovascularization model(OIR)was established in NLRP3 knockout(NLRP3-/-)mice and control homologous C57BL/6J mice. The samples were taken at P12, P17 and P21 days, and the neovascularization was observed by retinal fluorescence perfusion. The normal C57BL/6J mice that established the OIR model were selected and the samples were collected at P12, P17, and P21 days, and the retina was perfused with fluorescence. The OIR model was compared with the OIR model mice to verify the success of the establishment of the OIR model. The samples of the two groups of OIR mice were taken at P17, and the RNA level of VEGF was detected by RT-PCR to evaluate the regulation of NLRP3 deletion on VEGF expression. Results It was found that no perfusion area and neovascularization were found in the retina when the OIR model was not established in normal C57BL/6J, indicating that our OIR model was established successfully. Compared with OIR mice of C57BL/6J, the range of neovascularization in NLRP3-/- OIR mice was significantly reduced, the area of no perfusion was reduced, and the number of normal blood vessels was relatively increased in P12, P17 and P21. At P17, the VEGF expression level of NLRP3-/- OIR was significantly lower than that of normal OIR mice. Conclusion Through the study of retinal neovascularization in OIR mice, it was found that NLRP3 was involved in regulating the expression of VEGF and affecting the formation of retinal neovascularization. This study proposes a new pathway to regulate retinal neovascularization, which provides a new idea and target for the study and treatment of retinal vascular injury and neovascularization.

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备注/Memo

备注/Memo:
1 中山大学肿瘤防治中心内镜中心 华南肿瘤学国家重点实验室,通信作者,E-mail:huangchy@sysucc.org.cn
更新日期/Last Update: 2021-06-15