[1]王振河,姜德谦,许丹焰,等.可溶性环氧化物水解酶抑制剂对小鼠内皮祖细胞分泌血管内皮生长因子及缺氧诱导因子-1α的促进作用[J].福建医药杂志,2021,43(04):128-132.
　WANG Zhenhe,JIANG Deqian,XU Danyan,et al.Effects of soluble epoxide hydrolase inhibitors on secretion of vascular endothelial growth factor and hypoxia-inducible factor-1α by murine endothelial progenitor cells[J].FUJIAN MEDICAL JOURNAL,2021,43(04):128-132.
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可溶性环氧化物水解酶抑制剂对小鼠内皮祖细胞分泌血管内皮生长因子及缺氧诱导因子-1α的促进作用()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
43
期数:
2021年04期
页码:
128-132
栏目:
基础研究
出版日期:
2021-08-15

文章信息/Info

Title:
Effects of soluble epoxide hydrolase inhibitors on secretion of vascular endothelial growth factor and hypoxia-inducible factor-1α by murine endothelial progenitor cells
文章编号:
1002-2600(2021)04-0128-05
作者:
王振河; 姜德谦1; 许丹焰1; 李卫华; 谢强
厦门大学附属第一医院心内科（厦门 361003）
Author(s):
WANG Zhenhe;  JIANG Deqian;  XU Danyan;  LI Weihua;  XIE Qiang
Department of Cardiology, the First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, China
关键词:
可溶性环氧化物水解酶抑制剂; t-AUCB; 过氧化体增殖物激活型受体γ; 血管内皮生长因子; 缺氧诱导因子-1α; 内皮祖细胞
Keywords:
soluble epoxide hydrolase inhibitor; t-AUCB; catalosome proliferator-activated receptor γ; vascular endothelial growth factor; hypoxia-inducible factor-1α; endothelial progenitor cell
分类号:
R363
文献标志码:
A
摘要:
目的 探讨可溶性环氧化物水解酶抑制剂（t-AUCB）能否促进小鼠来源内皮祖细胞（EPCs）分泌内皮生长因子（VEGF）和缺氧诱导因子-1α（HIF-1α），及可能的相关机制。 方法 提取小鼠长骨骨髓，离心分离培养，取得高纯度EPCs，不同浓度可溶性环氧化物水解酶抑制剂（t-AUCB）和过氧化体增殖物激活型受体γ（PPAR-γ）阻断剂GW9662单独或联合干预EPCs，Western blot检测上述EPCs体外分泌VEGF及HIF-1α情况。结果从0～100 μmol/L，t-AUCB呈浓度依赖性增强EPCs体外分泌VEGF、HIF-1α能力，而5 μmol/L t-AUCB阻断剂GW9662可抑制EPC上述功能。1、10、50、100 μmol/L t-AUCB促进EPCs分泌VEGF、HIF-1α能力与0 μmol/L t-AUCB相比，差异均具有统计学意义（P<0.05）；GW9662+100 μmol/L t-AUCB与100 μmol/L t-AUCB比较，差异具有统计学意义（P<0.05），与0 μmol/L t-AUCB比较，差异也具有统计学意义（P<0.05）。 结论 t-AUCB可促进EPCs分泌VEGF及HIF-1α，其机制可能与激活PPAR-γ通路有关。
Abstract:
Objective To investigate whether soluble epoxide hydrolase inhibitors (t-AUCB) can promote the secretion of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α) in murine endothelial progenitor cells (EPCs), and the possible related mechanisms.Methods Long bone marrow of mice was extracted and centrifugally cultured to obtain high purity EPCs．Different concentrations of soluble epoxide hydrolase inhibitor (t-AUB) and peroxide proliferator-activated receptor γ (PPAR-γ) blocker GW9662 were used to interfere with EPCs alone or in combination.The secretion of VEGF and HIF-1α in vitro of EPCs was detected by Western blot.Results From 0 to 100 μmol/L, t-AUCB enhanced the ability of EPCs to secrete VEGF and HIF-1α in a concentration dependent manner, while 5 μmol/L t-AUCB blocker GW9662 inhibited the above functions of EPCs.Compared with 0 μmol/L t-AUCB, 1, 10, 50 and 100 μmol/L t-AUCB promoted the secretion of VEGF and HIF-1α in EPCs, and the differences were statistically significant (P<0.05).The difference between GW9662+100 μmol/L t-AUCB and 100 μmol/L t-AUCB was statistically significant (P<0.05), compared with 0 μmol/L t-AUCB, the difference was also statistically significant (P<0.05).Conclusion t-AUCB can promote the secretion of VEGF and HIF-1α in EPCs, and the mechanism may be related to the activation of PPAR-γ pathway.

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备注/Memo

备注/Memo:
基金项目：福建省卫生厅青年科研课题（2013-2-84）
1 中南大学湘雅二医院心内科（410011）
更新日期/Last Update: 2021-08-15