[1]章尤权,陈旭征,林瑶.松果菊苷对MPP+诱导的SH-SY5Y细胞氧化应激损伤的保护作用[J].福建医药杂志,2021,43(04):126-128.
　Zhang Youquan,Chen Xuzheng,LIN Yao.Protective effect of Echinoside on oxidative stress injury induced by MPP+ in SH-SY5Y cells[J].FUJIAN MEDICAL JOURNAL,2021,43(04):126-128.
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松果菊苷对MPP+诱导的SH-SY5Y细胞氧化应激损伤的保护作用 ()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
43
期数:
2021年04期
页码:
126-128
栏目:
基础研究
出版日期:
2021-08-15

文章信息/Info

Title:
Protective effect of Echinoside on oxidative stress injury induced by MPP+ in SH-SY5Y cells
文章编号:
1002-2600(2021)04-0126-03
作者:
章尤权; 陈旭征1; 林瑶2
福建中医药大学附属第二人民医院（福州 350001）
Author(s):
Zhang Youquan; Chen Xuzheng; LIN Yao
The Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou，Fujian 350122, China
关键词:
松果菊苷; 氧化应激; 细胞凋亡; 帕金森病
Keywords:
echinoside;  oxidative stress;  apoptosis;  parkinson's disease
分类号:
R741
文献标志码:
A
摘要:
目的 探讨松果菊苷(Echinacoside，ECH)对1-甲基-4-苯基吡啶离子（MPP+）诱导的人神经母细胞瘤细胞（SH-SY5Y)细胞氧化应激损伤的影响及机制。 方法 选取对数生长期的SH-SY5Y细胞分为对照组(正常培养，不给予任何试剂干预)、MPP+组(500 μmol/L MPP+处理细胞24 h)、ECH组(1、3、10、30、100 μmol/L ECH预处理细胞24 h后加500 μmol/L MPP+处理细胞24 h)。CCK8检测细胞存活率，倒置相差显微镜观察细胞形态，Hoechst33342荧光染色法观察凋亡细胞，化学比色法检测抗氧化酶SOD、CAT活力以及脂质过氧化物丙二醛MDA含量。 结果 与对照组相比，MPP+组细胞存活率显著下降；细胞生长状态明显变差；细胞内抗氧化酶SOD 和CAT活力显著下降，MDA 含量显著升高；出现明显细胞凋亡现象。松果菊苷预处理SH-SY5Y细胞24 h后，与MPP+组比较，细胞存活率显著上升；细胞生长状态明显好转；细胞内SOD、CAT活力显著上升，MDA含量明显减少；细胞凋亡现象明显缓解。 结论 松果菊苷对MPP+诱导氧化应激损伤的 SH-SY5Y 细胞具有保护作用，可通过增强细胞内源性抗氧化酶的活力、抑制脂质过氧化物的生成来抑制细胞凋亡。
Abstract:
Objective To investigate the effects of Echinacoside (ECH) on oxidative stress injury induced by MPP+ in SH->SY5Y cells and its mechanism．Methods SH-SY5Y cells in logarithmic growth phase were divided into control group (normal culture, without any reagent intervention), MPP+ group (500 μmol/L MPP+ treated cells for 24 h), and ECH group (1, 3, 10, 30, 100 μmol/L ECH pretreated cells for 24 h and 500 μmol/L MPP+ treated cells for 24h)．The survival rate of cells was determined by CCK8, and cell morphology was observed by inverted phase contrast microscope．The activities of antioxidant enzymes SOD and CAT and the content of lipid peroxide malondialdehyde (MDA) were determined by chemical colorimetry．The apoptotic cells were observed by Hoechst33342 fluorescence staining．Results Compared with the control group, the survival rate of MPP+ group was significantly decreased; the cell growth status was significantly worse．The activities of SOD and CAT in cells were significantly decreased, while the content of MDA was significantly increased．There was obvious apoptotic phenomenon．The survival rate of SH-SY5Y cells pretreated with ECH for 24 h was significantly higher than that of MPP+ group．The cell growth state was improved obviously．The activities of SOD and CAT increased significantly, while the content of MDA decreased significantly．Apoptosis was significantly alleviated．Conclusion ECH could protect SH-SY5Y cells from oxidative stress injury induced by MPP+, and inhibit cell apoptosis by enhancing the activity of endogenous antioxidant enzymes and inhibiting the generation of lipid peroxides.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金青年项目（81804156）；福建省自然科学基金项目（2018J01882）
1 福建中医药大学中西医结合研究院 福建省中西医结合老年性疾病重点实验室；
2 通信作者，福建中医药大学中西医结合学院， Email：55380098@qq.com
更新日期/Last Update: 2021-08-15