[1]张虹,张春,肖志勇.上皮膜蛋白1在鼻咽癌组织中的表达特征[J].福建医药杂志,2021,43(03):14-18.
　ZHANG Hong,ZHANG Chun,XIAO Zhiyong.Expressive features of epithelial membrane protein 1 in nasopharyngeal carcinoma[J].FUJIAN MEDICAL JOURNAL,2021,43(03):14-18.
点击复制

上皮膜蛋白1在鼻咽癌组织中的表达特征()
分享到：

《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
43
期数:
2021年03期
页码:
14-18
栏目:
临床研究
出版日期:
2021-06-15

文章信息/Info

Title:
Expressive features of epithelial membrane protein 1 in nasopharyngeal carcinoma
文章编号:
1002-2600(2021)03-0014-05
作者:
张虹; 张春1; 肖志勇
厦门大学附属福州市第二医院耳鼻咽喉头颈外科(福州 350007)
Author(s):
ZHANG Hong;  ZHANG Chun;  XIAO Zhiyong
Department of Otorhinolaryngology Head and Neck Surgery,the Affiliated Fuzhou Municipal Se-cond Hospital,Xiamen University,Fuzhou,Fujian 350007,China
关键词:
上皮膜蛋白1(EMP1);  鼻咽癌;  紧密连接
Keywords:
epithelial membrane protein 1(EMP1);  nasopharyngeal carcinoma;  tight junction
分类号:
R739.63
文献标志码:
A
摘要:
目的 探讨上皮膜蛋白1(EMP1)在鼻咽癌组织与正常鼻黏膜组织中的表达差异及其与疾病发展的关系。方法 收集40例鼻咽癌患者的癌组织(实验组)与正常鼻黏膜组织(对照组)各40份标本,RT-qPCR、免疫荧光染色和免疫印记技术检测EMP1在两种组织中的表达情况; 40例鼻咽癌标本免疫印迹测定EMP1灰度值与其对应的肿瘤分化程度、临床分期进行二元线性回归分析。结果 与正常鼻黏膜上皮对比,鼻咽癌上皮细胞的EMP1表达明显降低(P<0.05),肿瘤细胞EMP1的表达与肿瘤分化程度呈正相关(P<0.05),与临床分期不存在线性关系(P>0.05)。结论 EMP1在鼻咽癌细胞和正常鼻黏膜细胞表达有差异,其值可能预示肿瘤分化程度。
Abstract:
Objective To investigate the expressive differences of epithelial membrane protein 1(EMP1)between nasopharyngeal carcinoma tissues and normal nasal mucosa tissues and its relationship with disease development.Methods The expression of EMP1 in 40 nasopharyngeal carcinoma tissues(experimental group)and normal nasal mucosa tissues(control group)were detected by RT-qPCR, immunofluorescence staining and immunoblotting.The gray value of EMP1 was determined by Western blot in 40 nasopharyngeal carcinoma specimens, and the binary linear regression analysis was performed with tumor differentiation degree and clinical stage.Results Compared with normal nasal mucosa epithelium cells, the expression of EMP1 in nasopharyngeal carcinoma epithelial cells was significantly decreased(P<0.05).The expression of EMP1 in tumor cells was positively correlated with the degree of tumor differentiation(P<0.05),but there was no significant linear relationship between EMP1 expression and clinical stage(P>0.05).Conclusion The expression of EMP1 in nasopharyngeal carcinoma cells is di-fferent from that in normal nasal mucosa cells, which may predict the degree of tumor differentiations.

参考文献/References:

[1] 梁锌,杨剑,高婷,等.中国鼻咽癌流行情况[J].中国肿瘤,2016,25(11):835-840.
[2] Petersson F.Nasopharyngeal carcinoma:a review[J]. Semin Diagn Pathol,2015,32(1):54-73.
[3] Huang C L,Guo R,Li J Y,et al.Nasopharyngeal carcinoma treated with intensity-modulated radiotherapy: clinical outcomes and patterns of failure among subsets of 8th AJCC stage IVa [J].Eur Radiol,2020,30(2):816-822.
[4] Busch M, Klein S, Groβe-kreul J, et al.p53, miR-34a and EMP1-newly identified targets of TFF3 signaling in Y79 retinoblastoma cells[J].Int J Mol Sci, 2019,20(17):E4129.
[5] Wang Y W, Cheng H L, Ding Y R, et al.EMP1, EMP 2, and EMP3 as novel therapeutic targets in human cancer[J]. Biochim Biophys Acta Rev Cancer, 2017,1868(1):199-211.
[6] 丛馨,张艳,俞光岩,等.上皮细胞间紧密连接的研究进展[J].生理学报,2016,68(4):492-504.
[7] Taylor V,Welcher A A,Program A E,et al.Epithelial membrane protein-1, peripheral myelin protein 22, and lens membrane protein 20 define a novel gene family[J]. Biol Chem, 1995, 270(48):28824-28833.
[8] Wulf P, Suter U.Embryonic expression of epithelial membrane protein 1 in early neurons[J]. Brain Res Dev Brain Res, 1999, 116(2):169-180.
[9] Sun G G,Zhao G,Lu Y F,et al.Association of EMP1 with gastric carcinoma invasion,sruvival and prognosis[J].Int J Oncol, 2014, 45(3): 1091-1098.
[10] Li H,Zhang X,Jiang X,et al.The expression and function of epithelial membrane protein 1 in laryngeal carcinoma[J].Int J Oncol,2017,50(1):141-148.
[11] 代晓华,张军,邹慧儒,等.上皮膜蛋白1真核表达载体的构建及其对人舌鳞状细胞癌细胞迁移和侵袭对影响[J].华西口腔医学杂志,2016,34(4):398-403.
[12] Liu Y,Ding Y L,Nie Y T,et al.EMP1 promotes the proliferation and invasion of ovarian cancer cells through activating the MAPK pathway[J].Onco Targets and Therapy,2020,13:2047-2055.
[13] Lin B,Zhang T,Ye X,et al.High expression of EMP1 predicts a poor prognosis and correlates with immune infiltrates in bladder urothelial carcinoma[J].ONcol Lett,2020,20(3):2840-2854.
[14] Echevarria M I,Awasthi S,Cheng C H,et al.African American specific gene panel predictive of poor prostate cancer outcome[J].J Urol,2019,202(2):247-255.
[15] Bangsow T,Baumann E,Bangsow C,et al.The epithelial membrane protein 1 is a novel tight junction protein of the blood-brain barrier[J].J Cereb Flow Metab,2008,28(6):1249-1260.
[16] Tzanakakis G,Kavasi R M,Voudouri K,et al.Role of the extracellular matrix in cancer-associated epithelial to mesenchymal transition phenomenon[J].Developmental Dynamics,2018,247(3):368-381.
[17] Durgan J,Tao G B,Walters M S,et al.SOS1 and Ras regulate epithelial tight junction formation in the human airway through EMP1[J].EMBO Rep,2015,16(1):87-96.

备注/Memo

备注/Memo:
1 福建医科大学附属福建省肿瘤医院头颈放疗科
更新日期/Last Update: 2021-06-15