[1]余炜,黄静,余辉,等.基于介孔二氧化硅和聚多巴胺纳米载体递送阿帕替尼的性质研究[J].福建医药杂志,2024,46(02):114-118.[doi:10.20148/j.fmj.2024.02.031]
　YU Wei,HUANG Jing,YU Hui,et al.Study on the properties of apatinib delivery based on mesoporous silica and polydopamine nanocarriers[J].FUJIAN MEDICAL JOURNAL,2024,46(02):114-118.[doi:10.20148/j.fmj.2024.02.031]
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基于介孔二氧化硅和聚多巴胺纳米载体递送阿帕替尼的性质研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年02期
页码:
114-118
栏目:
基础研究
出版日期:
2024-04-15

文章信息/Info

Title:
Study on the properties of apatinib delivery based on mesoporous silica and polydopamine nanocarriers
文章编号:
1002-2600(2024)02-0114-05
作者:
余炜1; 黄静1; 余辉1; 魏丞2; 范芳1; 林海燕1; 林剑扬1; 翁金森3
1 福建医科大学肿瘤临床医学院 福建省肿瘤医院临床药学室,福州 350014; 2 胃肠外科,福州 350014; 3 重症医学科,福州 350014
Author(s):
YU Wei1; HUANG Jing1; YU Hui1; WEI Cheng2; FAN Fang1; LIN Haiyan1; LIN Jianyang1; WENG Jinsen3
1 Department of Clinical Pharmacy, Fujian Cancer Hospital, Clinical Oncology School of Fujian Medical University, Fuzhou, Fujian 350122, China; 2 Department of Gastrointestinal Surgery, Fujian Cancer Hospital, Clinical Oncology School of Fujian Medical University, Fuzhou, Fujian 350122, China; 3 Department of Critical Care Medicine, Fujian Cancer Hospital, Clinical Oncology School of Fujian Medical University, Fuzhou, Fujian 350122, China
关键词:
抗肿瘤;  阿帕替尼;  MSNs@PDA-Apa;  纳米粒子
Keywords:
antitumor;  apatinib;  MSNs@PDA-Apa;  nanoparticle
分类号:
R943
DOI:
10.20148/j.fmj.2024.02.031
文献标志码:
B
摘要:
目的 探讨基于介孔二氧化硅和聚多巴胺的纳米载体递送阿帕替尼的效能。方法 利用介孔二氧化硅(MSN)的高效药物递送能力和聚多巴胺(PDA)的pH响应性,采用改进的Stober法制备了介孔二氧化硅纳米粒子,根据静电复合法设计了一种pH响应性聚多巴胺(PDA)包覆的介孔二氧化硅纳米给药系统(MSNs@PDA)。采用扫描电子显微镜(SEM)和Zeta电位改变验证MSNs@PDA系统制备是否成功。测定MSNs@PDA-Apa的载药量和包埋率。CCK-8法评价体外细胞毒性。Transwell法评价细胞迁移。结果 扫描电子显微镜(SEM)和Zeta电位改变验证MSNs@PDA-Apa制备成功。MSNs@PDA-Apa的药物载药量为2.4%,包埋率为48%±2.5%。MSNs@PDA-Apa组的细胞毒性呈浓度依赖性,当浓度达到10 μg/mL时,PC-9细胞的存活率仅为30%左右。与Apa组相比,MSNs@PDA-Apa组对细胞迁移的效果明显增强。结论 MSNs@PDA-Apa具有较高的药物传递效率,表现出对肿瘤细胞的良好杀伤作用,有望为未来的药物传递系统的构筑提供思路和方法。
Abstract:
Objective To explore the efficacy of nanocarriers based on mesoporous silica and polydopamine for delivering apatinib.Methods By utilizing the efficient drug delivery ability of mesoporous silica(MSN)and the pH responsiveness of polydopamine(PDA), mesoporous silica nanoparticles were prepared using an improved Stober method.A pH responsive polydopamine(PDA)coated mesoporous silica nanodrug delivery system was designed based on electrostatic composite method(MSNs@PDA).The success of MSNs@PDA system preparation was verified by scanning electron microscopy(SEM)and Zeta potential changes.The drug loading capacity and embedding rate of MSNs@PDA-Apa were determined.Vitro cytotoxicity was evaluated by the CCK-8 method.Transwell method was used to evaluate cell migration.Results MSNs@PDA system preparation was successful, which was verified by scanning electron microscopy(SEM)and Zeta potential changes.The drug loading capacity of MSNs@PDA-Apa was 2.4%, and the embedding rate was 48%±2.5%.The cytotoxicity of MSNs@PDA-Apa group was concentration dependent, and when the concentration reached 10 μg/mL, the survival rate of PC-9 cells was only about 30%.Compared to the Apa group, the effect of MSNs@PDA-Apa group on cell migration was significantly enhanced.Conclusion MSNs@PDA-Apa system preparation has a high drug delivery efficiency and exhibits a good killing effect on tumor cells, which is expected to provide ideas and methods for the construction of future drug delivery systems.

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金项目(2020J011119)
通信作者:翁金森,Email:fjzzyxk@163.com
更新日期/Last Update: 2024-04-15