[1]黄建刚,沈菁,陈雯,等.长链非编码RNA AC004920.3在胃腺癌癌栓和细胞中的作用[J].福建医药杂志,2024,46(01):5-8.[doi:10.20148/j.fmj.2024.01.002]
　HUANG Jiangang,SHEN Jing,CHEN Wen,et al.Effects of long non-coding RNA AC004920.3 on cancer embolus and cells of gastric adenocarcinoma[J].FUJIAN MEDICAL JOURNAL,2024,46(01):5-8.[doi:10.20148/j.fmj.2024.01.002]
点击复制

长链非编码RNA AC004920.3在胃腺癌癌栓和细胞中的作用()
分享到：

《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年01期
页码:
5-8
栏目:
临床研究
出版日期:
2024-02-15

文章信息/Info

Title:
Effects of long non-coding RNA AC004920.3 on cancer embolus and cells of gastric adenocarcinoma
文章编号:
1002-2600（2024）01-0005-04
作者:
黄建刚1; 沈菁2; 陈雯2; 林有东2
1 福建省临床检验中心，福州 350001；2 福建省立医院检验科，福州 350001
Author(s):
HUANG Jiangang1;  SHEN Jing2;  CHEN Wen2;  LIN Youdong2
1 Fujian Provincial Center for Clinical Laboratory, Fuzhou, Fujian 350001, China; 2 Department of Clinical Laboratory Medicine, Fujian Provincial Hospital, Fuzhou, Fujian 350001, China
关键词:
AC004920.3; 胃腺癌; 脉管癌栓; 细胞凋亡; 细胞增殖; 细胞迁移
Keywords:
AC004920.3;  gastric adenocarcinoma; vascular cancer thrombus; cell apoptosis; cell proliferation; cell migration
分类号:
R319；R737.3
DOI:
10.20148/j.fmj.2024.01.002
文献标志码:
A
摘要:
目的探寻AC004920.3在胃腺癌患者血清中的表达和细胞中的作用。方法收集胃腺癌患者血清标本和病历资料。PCR扩增AC004920.3的表达。慢病毒过表达AC004920.3转染AGS细胞后进行凋亡、CCK增殖和划痕实验。结果胃腺癌脉管癌栓患者血清AC004920.3表达ΔCt值12.06±3.33明显高于无脉管癌栓ΔCt值的10.05±3.32，差异有统计学意义（P=0.003），与D-二聚体呈正相关（r=0.679,P<0.001）。AC004920.3过表达组AGS细胞早期凋亡率为（33.45±3.90）%，高于未转染组[（12.13±1.54）%,P<0.001]和空载体组[（13.18±1.55）%，P<0.001]；CCK增殖实验AC004920.3过表达组的A值为2.89±0.17，低于未转染组的3.20±0.21，差异有统计学意义（P=0.003）；也低于空载体组3.28±0.18，差异有统计学意义（P=0.001)；AC004920.3过表达组的划痕面积（3.54±0.12）灰度值，明显大于未转染组和空载体组（2.43±0.34）灰度值，差异有统计学意义（P=0.001）；也大于空载体组（2.60±0.39）灰度值，差异有统计学意义（P=0.004）。结论胃腺癌脉管癌栓患者血清AC004920.3高表达，且与D-二聚体正相关。AC004920.3促进AGS细胞凋亡以及抑制AGS细胞增殖和迁移。
Abstract:
ObjectiveTo investigate the expression of AC004920.3 in serum of gastric adenocarcinoma patients and its effect on cells.MethodsSerum samples and medical records were collected.The expression of AC004920.3 was amplified by PCR.After transfecting AGS cells with lentivirus overexpression of AC004920.3, apoptosis, CCK proliferation and scratches were performed.ResultsSerum AC004920.3 expression 12.06±3.33 was significantly lower in patients with gastric adenocarcinoma with venous embolus than that without venous embolus(10.05±3.32, P=0.003), and was positively correlated with D-dimer(r=0.679, P<0.001).The early apoptosis rate of AGS cells in AC004920.3 overexpression group(33.45±3.90)% was higher than that in untransfected group [(12.13±1.54）%,P<0.001] and transfection negative control group[(13.18±1.55）%，P<0.001].The proliferation of CCK showed that OD value of AC004920.3 overexpression group(2.89±0.17) was lower than that of untransfected group and transfection negative control group(3.20±0.21, P=0.003; 3.28±0.18, P=0.001). The scratch area of AC004920.3 overexpression group(3.54±0.12) was significantly larger than that of untransfected group and transfection negative control group(2.43±0.34, P=0.001; 2.60±0.39, P=0.004).ConclusionThe serum AC004920.3 expression in patients with gastric adenocarcinoma with venous embolus was lower than that without venous embolus, and was positively correlated with D- dimer.AC004920.3 promoted the apoptosis of AGS cells and inhibited the proliferation and migration of AGS cells.

参考文献/References:

[1] XIA C,DONG X,LI H,et al.Cancer statistics in China and United States,2022：profiles,trends,and determinants[J].Chin Med J(Engl),2022,135(5):584-590.
[2] 周家琛,魏文强,赫捷,等.2000—2015年中国肿瘤登记地区胃癌发病趋势及年龄变化[J].实用肿瘤学杂志,2020,34(1):1-5.
[3] YUAN L,XU Z Y,RUAN S M,et al.Long non-coding RNAs towards precision medicine in gastric cancer：early diagnosis,treatment,and drug resistance[J].Mol Cancer,2020,19(1):96.
[4] KRAMER S J,AMANAVICIUS N,STAANUM P F,et al.Incidental finding of pulmonary embolism at workup before SIRT[J].Clin Nucl Med,2022,47(2):e201-e202.
[5] LUO Z,MA G,LU Y,et al.Characteristics and 6-Month mortality of medical oncology patients with incidental and symptomatic pulmonary embolism：A single institutional retros-pective longitudinal analysis[J].Clin Appl Thromb Hemost,2023,29:10760296231155177.
[6] MAHé I,CHAPELLE C,PLAISANCE L,et al.Management of cancer-associated thrombosis in France：A national survey among vascular disease and supportive care specialists[J].Cancers(Basel),2022,14(17):4143.
[7] HOUGHTON D E,VLAZNY D T,CASANEGRA A I,et al.Bleeding in patients with gastrointestinal cancer compared with nongastrointestinal cancer treated with apixaban,rivaroxaban,or enoxaparin for acute venous thromboembolism[J].Mayo Clin Proc,2021,96(11):2793-2805.
[8] WANG W D,WEI J P,GAO R Q,et al.A retrospective study of the role of preoperative ultrasonography in the detection of deep vein thrombosis in 1750 patients with gastric and colorectal cancers[J].Thromb Res,2022,216:52-58.
[9] KIM E,KIM H,YEO M K,et al.Identification of a novel long non-coding RNA,lnc-ATMIN-4:2,and its clinicopathological and prognostic significance in advanced gastric cancer[J].Cancer Genomics Proteomics,2022,19(6):761-772.
[10] ZhANG N,WANG B,MA C,et al.LINC00240 in the 6p22.1 risk locus promotes gastric cancer progression through USP10-mediated DDX21 stabilization[J].J Exp Clin Cancer Res,2023,42(1):89.
[11] ASHRAFIZADEH M,MOHAN C D,RANGAPPA S,et al.Noncoding RNAs as regulators of STAT3 pathway in gastrointestinal cancers：Roles in cancer progression and therapeutic response[J].Med Res Rev,2023 43(5):1263-1321.
[12] DANG Y,OUYANG X,ZHANG F,et al.Circular RNAs expression profiles in human gastric cancer[J].Sci Rep,2017,7(1):9060.
[13] 王昊,袁建松.消化道恶性肿瘤并发静脉血栓栓塞的分子机制与风险评估[J].中国分子心脏病学杂志,2021,21(6):4380-4383.
[14] 段炼,何国琳.妊娠期D-二聚体升高带来的临床困惑[J].四川大学学报(医学版),2022,53(3):543-548.
[15] YU H,QIN X K,YIN K W,et al.EphB6 deficiency in intestinal neurons promotes tumor growth in colorectal cancer by neurotransmitter GABA signaling[J].Carcinogenesis,2023,44(9)：682-694.
[16] HANOVER G,VIZEACOUMAR F S,BANERJEE S L,et al.Integration of cancer-related genetic landscape of Eph receptors and ephrins with proteomics identifies a crosstalk between EPHB6 and EGFR[J].Cell Rep,2023,42(7):112670.
[17] LIU J,XU B,XU G,et al.Reduced EphB6 protein in gastric carcinoma and associated lymph nodes suggests EphB6 as a gastric tumor and metastasis inhibitor[J].Cancer Biomark,2017,19(3):241-248.

备注/Memo

备注/Memo:
基金项目：福建省卫健委医学创新课题(2018007)
通信作者：林有东，Email：2444734512@qq.com
更新日期/Last Update: 2024-02-15