[1]张璇,章斌斌,陈仔颖.基于“3A学说”探讨逐瘀汤对子宫内膜异位症大鼠VEGF表达的影响[J].福建医药杂志,2022,44(01):1-4.
　ZHANG Xuan,ZHANG Binbin,CHEN Zaiying.Effect of Zhuyu decoction on the VEGF levels of rats with endometriosis based on the 3A theory[J].FUJIAN MEDICAL JOURNAL,2022,44(01):1-4.
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基于“3A学说”探讨逐瘀汤对子宫内膜异位症大鼠VEGF表达的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期数:
2022年01期
页码:
1-4
栏目:
临床研究
出版日期:
2022-02-15

文章信息/Info

Title:
Effect of Zhuyu decoction on the VEGF levels of rats with endometriosis based on the 3A theory
文章编号:
1002-2600(2022)01-0001-04
作者:
张璇; 章斌斌; 陈仔颖
福建中医药大学附属人民医院(福州350004)
Author(s):
ZHANG Xuan;  ZHANG Binbin;  CHEN Zaiying
The Affiliated People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, China
关键词:
逐瘀汤; 子宫内膜异位症大鼠; VEGF
Keywords:
Zhuyu decoction;  endometriosis;  VEGF
分类号:
R711.71
文献标志码:
A
摘要:
目的 基于“3A学说”探讨逐瘀汤对子宫内膜异位症(EMS)大鼠VEGF的影响。方法 采用自体移植方法建立EMS模型，将建模成功的大鼠采用随机数字表法分为对照组、米非司酮组及逐瘀汤组，选取正常饲养SD大鼠作为假手术组，每组20只。采用ELISA法检测各组大鼠治疗前、治疗后血清VEGF水平;采用Western blot法检测各组腹腔移植物组织VEGF蛋白表达情况;采用RT-PCR法检测各组腹腔移植物组织VEGF mRNA表达情况。结果 1)治疗前与假手术组比较，其他3组大鼠血清中VEGF水平显著升高(P<0.05); 治疗后，米非司酮组、逐瘀汤组大鼠血清中VEGF水平较对照组显著降低(P<0.05);米非司酮组与逐瘀汤组大鼠血清VEGF水平比较，差异无统计学意义(P>0.05)。 2)治疗后米非司酮组与逐瘀汤组大鼠腹腔移植物组织VEGF蛋白表达较对照组明显降低(P<0.05)，米非司酮组与逐瘀汤组表达差异无统计学意义(P>0.05)。 3)治疗后，与对照组相比，米非司酮组和逐瘀汤组大鼠异位内膜组织VEGF mRNA表达水平均明显降低(P<0.05), 米非司酮组与逐瘀汤组表达差异无统计学意义(P>0.05)。结论 逐瘀汤 能降低EMT大鼠血清中VEGF水平，可以减轻异位内膜组织中VEGF蛋白及mRNA的表达。
Abstract:
Objective To explore the effect of Zhuyu decoction on the VEGF levels in rats with endometriosis based on the 3A theory. Methods By autologous transplantation, the EMS rats models were successfully established. These models were randomly divided into model control group, mifepristone group and Zhuyu decoction group through method of random number table. Normal feeding SD rats were selected as sham operation group. Each group included 20 rats. The serum levels of VEGF of each group were tested through ELISA before and after treatment. The levels of VEGF expression in endometriosis were detected by Western blot. And the level of VEGF expression in ectopic tissues were detected by Fluorescence quantitative PCR. Results ①Compared with the sham operation group, the serum levels of VEGF in other groups were significantly increased before treatment (P<0. 05). After treatment, the serum levels of VEGF in the mifepristone group and the Zhuyu decoction group were significantly lower, compared with the model control group (P<0.05). There was no significant difference in the serum level of VEGF between the mifepristone group and the Zhuyu Decoction group (P>0.05).②After treatment, the protein expression of VEGF in the abdominal graft tissue in the mifepristone group and the Zhuyu decoction group was lower than that in the control group (P<0.05)，and the protein expression of mifepristone group and Zhuyu decoction group was not significantly different (P>0.05).③After treatment, the serum level of VEGF gene expression in the mifepristone group and the Zhuyu decoction group was lower than that in the control group (P<0.05)，there was no significant difference in the serum level of VEGF gene expression bet ween the mifepristone group and the Zhuyu decoction group (P>0.05). Conclusion Zhuyu decoction can reduce the serum VEGF of rats with endometriosis, thus reduce the protein and mRNA expression of VEGF.

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相似文献/References:

[1]张璇 王小红 林妍 吴小玲.逐瘀汤对血瘀型子宫内膜异位症性不孕的疗效观察[J].福建医药杂志,2017,39(5):101.

备注/Memo

备注/Memo:
基金项目:福建省自然科学基金资助项目(2018J01332)
更新日期/Last Update: 2022-02-15