[1]许少华 周 东 张 辉 陈昭硕.慢病毒介导的靶向SNCG敲除对人结肠癌SW1116裸鼠结肠癌肝转移 实验模型的影响[J].福建医药杂志,2019,41(05):120-123.
　XU Shaohua,ZHOU Dong,ZHANG Hui,et al.The effect of the lentivirus-mediated RNA interference targeting SNCG gene on the growth of human colorectal carcinoma cell SW1116 in nude mice[J].FUJIAN MEDICAL JOURNAL,2019,41(05):120-123.
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慢病毒介导的靶向SNCG敲除对人结肠癌SW1116裸鼠结肠癌肝转移 实验模型的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
41
期数:
2019年05期
页码:
120-123
栏目:
基础研究
出版日期:
2019-10-15

文章信息/Info

Title:
The effect of the lentivirus-mediated RNA interference targeting SNCG gene on the growth of human colorectal carcinoma cell SW1116 in nude mice
文章编号:
1002-2600(2019)05-0120-04
作者:
许少华 周 东1 张 辉 陈昭硕
福建医科大学附属肿瘤医院 福建省肿瘤医院(福州 350001)
Author(s):
XU Shaohua;  ZHOU Dong; ZHANG Hui;  CHEN Zhaoshuo
Fujian Provincial Tumor Hospital, the Affiliated Tumor Hospital of Fujian Medical University,Fuzhou,Fujian 350014,China
关键词:
慢病毒载体;  SNCG基因;  基因敲除;  裸鼠;  结肠癌肝转移
Keywords:
lentiviral vector;  SNCG Gene;  RNA interference;  nude mice;  hepatic metastasis of colon carcinoma
分类号:
R735
文献标志码:
A
摘要:
目的 研究慢病毒介导的γ突触核蛋白(SNCG)基因敲除对人结肠癌SW1116细胞在裸鼠结肠癌肝转移实验模型中的影响。方法 前期实验中,通过SNCG基因敲除并慢病毒转染,构建稳定表达的SNCG敲除实验组(RNAi组)、转染空质粒的空质粒对照组(NC组)。复苏基因敲除实验组(RNAi组)、空质粒对照组(NC组)及空白对照组(CON组)的野生型SW1116细胞,保脾法建立裸鼠结肠癌肝转移模型,免疫组化染色、逆转录聚合酶链反应(RT-PCR)、蛋白质免疫印迹(Western blot)检测SNCG基因的表达。结果 1)成功构建裸鼠结肠癌肝转移模型。2)与对照组比较,RNAi组裸鼠移植瘤生长缓慢,原位成瘤率及肝转移率均明显下降。3)免疫组化、RT-PCR及Western blot结果显示,RNAi组SNCG mRNA和蛋白表达均明显下降。结论 SNCG基因沉默后,裸鼠脾脏原位移植瘤及转移瘤的数量变少,体积变小。在体内,靶向SNCG敲除降低了原位移植瘤及肝脏转移瘤中SNCG基因的mRNA及蛋白表达,表明SNCG基因对结肠癌的增生、侵袭起积极推动作用。利用RNAi技术抑制SNCG基因的表达有望为结肠癌的基因治疗提供新靶点、新手段。
Abstract:
Objective To study the effect of the lentivirus-mediated RNA interference targetingγ-synuclein(SNCG)gene on the growth of human colorectal carcinoma cell SW1116 in vivo of nude mice.Methods In earlier experiment, we first to screen SNCG RNAi effective target genes, then, transfected and packaged by the lentiviral vectors.Recombinant lentiviral vectors respectively carrying the SNCG-RNAi and SNCG-NC were successfully constructed.The SNCG-RNAi(RNAigroup), SNCG-NC(NCgroup), and non-transfected Human colorectal carcinoma SW1116 cells(CON group),were thawed.And then the colon cancer liver metastasis model in nude mice was established.In order to find outthe difference, SNCG gene expression in these tumor issues were detected by Immunohistochemistry assay, RT-PCR and Western blot.Results 1)The colon cancer liver metastasis model in nude mice was successfully established.2)Compared with those in NC group and CON group, the tumor in the RNAi group grew slowly and metastasis was evidentlysuppressed.3)The mRNA and protein expression of SNCG of the tumor in the RNAi group were significantly lower according to theIHC result, RT-PCR and Western blot.Conclusion Recombinant lentivirus vector SNCG-RNAi decreased the growth and quantity of the tumor in the colon cancer liver metastasis model,including the primary and metastatic, and it can obviously inhibit SNCG gene mRNA and protein expression.So it prompt that the expression of SNCG gene promoted colorectal cancer to grow and transfer.SNCG is expected to become a potent target for the treatment of colon cancer.

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更新日期/Last Update: 2019-10-15