[1]陈璐璐 翁 恒 李红艳 潘建光 黄艳生 钟艳芬.人肺泡灌洗液巨噬细胞吞噬功能和血清巨噬细胞集落刺激因子 抗体检测水平对肺泡蛋白沉积症的临床意义(附51例分析)[J].福建医药杂志,2019,41(01):9-12.
　CHEN Lulu,WENG Heng,LI Hongyan,et al.Clinical observation of human alveolar macrophages combined with serum granulocyte macrophage colony stimulating factor autoantibody(GMAbs)level on patients with pulmonary alveolar proteinosis[J].FUJIAN MEDICAL JOURNAL,2019,41(01):9-12.
点击复制

人肺泡灌洗液巨噬细胞吞噬功能和血清巨噬细胞集落刺激因子 抗体检测水平对肺泡蛋白沉积症的临床意义(附51例分析)()
分享到：

《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
41
期数:
2019年01期
页码:
9-12
栏目:
临床研究
出版日期:
2019-01-20

文章信息/Info

Title:
Clinical observation of human alveolar macrophages combined with serum granulocyte macrophage colony stimulating factor autoantibody(GMAbs)level on patients with pulmonary alveolar proteinosis
文章编号:
1002-2600(2019)01-0009-04
作者:
陈璐璐 翁 恒 李红艳1 潘建光 黄艳生 钟艳芬
福建省福州肺科医院(福州 350009)
Author(s):
CHEN Lulu;  WENG Heng;  LI Hongyan;  PAN Jianguang;  HUANG Yansheng;  ZHONG Yanfeng.
Department of Respiratory Diseases, Fuzhou Pulmonary Hospital of Fujian, Fuzhou, Fujian 350008, China
关键词:
肺泡蛋白沉积症;  肺泡巨噬细胞;  白介素-1β
Keywords:
pulmonary alveolar proteinosis;  alveolar macrophages;  interleukin-1β
分类号:
R563
文献标志码:
B
摘要:
目的 定量测肺泡巨噬细胞数量及肺泡巨噬细胞分泌白介素-1β水平,探讨特发性肺泡蛋白沉积症(PAP)患者肺泡巨噬细胞功能在其诊断及监测中的意义。方法 连续收集呼吸科2010年4月至2017年12月就诊的51例PAP患者。纳入同期47例其他弥漫性间质性肺疾病的住院患者作为对照组,所有患者收集肺泡灌洗液及血清标本,通过血清巨噬细胞集落刺激因子抗体(GMAbs)水平检测,进一步明确PAP患者,以ELISA方法测定肺泡灌洗液巨噬细胞分泌白介素-1β水平,同时计数肺泡灌洗液巨噬细胞数量。结果 所有入选PAP患者均行血清巨噬细胞集落刺激因子抗体水平检测,结果均呈阳性。患者肺泡灌洗液巨噬数量明显低于对照组(P<0.01),但疾病不同严重程度两组比较差异无统计学意义。肺泡巨噬细胞分泌IL-1β水平明显低于对照组,差异具有统计学意义(P<0.01)。结论 PAP患者肺泡灌洗液巨噬细胞数量及肺泡巨噬细胞分泌IL-1β水平明显低于对照组,间接反映了PAP患者巨噬细胞数量及功能明显减低。
Abstract:
Objective The number of alveolar macrophages and the level of interleukin-1 beta secreted by alveolar macrophages were measured quantitatively to explore the significance of alveolar macrophage function in the diagnosis and monitoring of idiopathic pulmonary alveolar proteinosis(PAP). Methods Over seven years, 51 patients with PAP were diagnosed at our hospital and enrolled in the study. 47 patients with other diffuse interstitial lung diseases in the same period were included as control group. Alveolar lavage fluid and serum samples were collected from all patients. The level of serum macrophage colony stimulating factor antibody(GMAbs)was detected to further identify PAP patients. The level of interleukin-1 beta secreted by alveolar lavage fluid macrophages was determined by ELISA, and the number of alveolar lavage fluid macrophages was counted. Results All the selected patients with pulmonary alveolar proteinosis were tested for serum granulocyte macrophage colony stimulating factor autoantibody(GMAbs), and the results were positive. The number of macrophages in alveolar lavage fluid was significantly lower than that in the control group(P<0.01), but there was no significant difference between the two groups in different severity of the disease. The level of IL-1 beta secretion in alveolar macrophages was significantly lower than that in disease control group(P<0.01). Conclusion The number of macrophages in alveolar lavage fluid and the level of IL-1beta secreted by alveolar macrophages in PAP patients are significantly lower than those in the control group, which indirectly indicates that the number and function of macrophages in PAP patients decrease.

参考文献/References:

[1] Shah P L, Hansell D, Lawson P R, et al. Pulmonary alveolar proteinosis: clinical aspects and current concepts on pathogenesis[J].Thorax,2000, 55(1):67-77.
[2] Seymour J F, Presneill J J. Pulmonary alveolar proteinosis: progress in the first 44 years [J].Am J Respir Crit Care Med,2002,166(2):215-235.
[3] Suzuki T, Trapnell B C. Pulmonary alveolar proteinosis syndrome[J].Clin Chest Med,2016, 37(3):431-440.
[4] Xu Z, Jing J, Wang H, et al. Pulmonary alveolar proteinosis in China: a systematic review of 241 cases[J].Respirology,2009,14(5):761-766.
[5] 徐凯峰,陈燕,郭子健,等.肺泡蛋白沉积症患者血清中抗粒-巨噬细胞集落刺激因子抗体等血清学指标的临床意义[J].中华结核和呼吸杂志,2004,27(12): 824-828.
[6] 李妍,田欣伦,桂耀松,等.自身免疫性肺泡蛋白沉积症血清脂代谢持点[J].中国医学科学院学报,2014,11(6):645-649.
[7] Inoue Y, Trapnell B C, Tazawa R, et al. Characteristics of a large cohort of patients with autoimmune pulmonary alveolar proteinosis in Japan[J].Am J Respir Crit Care Med, 2008, 177(7):752-762.
[8] Arai T, Inoue Y, Sugimoto C, et al. Cyfra 21-1 as a disease severity marker for autoimmune pulmonary alveolar proteinosis[J].Respirology,2014, 19(2):246-252.
[9]Campo I,Mariani F,Rodi G,et al.Assessment and management of pulmonary alveolar proteinosis in a reference center[J].Orphanel J Rare Dis,2013,45(8):40-45.
[10] Vercauteren I M,Verleden S E,McDonough J E,et a1.CYFRA 21-1 in bronchoalveolar lavage of idiopathic pulmonary fibrosis patients[J].Exp Lung Res,2015,41(8):459-465.
[11] Long X, He X, Ohshimo S, et al. Serum YKL-40 as predictor of outcome in hypersensitivity pneumonitis[J].Eur Respir J,2017,49(2):459-465.
[12] Malur A, Baker A D, McCoy A J, et al. Restoration of PPARgamma reverses lipid accumulation in alveolar macrophages of GM-CSF knockout mice[J].Am J Physiol Lung Cell Mol Physiol,2011,30(1):73-80.

相似文献/References:

[1]黄建忠,张文清,黄巧文,等.肺泡蛋白沉积症行大容量单侧肺灌洗术的麻醉处理[J].福建医药杂志,2017,39(4):143.

备注/Memo

备注/Memo:
基金项目:福州市科技计划项目(2015-S-142-3) 1 通信作者,Email:1353626039@qq.com
更新日期/Last Update: 2019-01-20