[1]李露,张令歌,张乔娜,等.壳多糖酶-3样蛋白1在终末期肾病患者失功自体动静脉内瘘组织中的表达及作用机制[J].福建医药杂志,2024,46(08):5-9.[doi:10.20148/j.fmj.2024.08.002]
　LI Lu,ZHANG Lingge,ZHANG Qiaona,et al.Expression of CHI3L1 in failed autologous arteriovenous fistula of patients with end-stage renal disease and its mechanism of action[J].FUJIAN MEDICAL JOURNAL,2024,46(08):5-9.[doi:10.20148/j.fmj.2024.08.002]
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壳多糖酶-3样蛋白1在终末期肾病患者失功自体动静脉内瘘组织中的表达及作用机制()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年08期
页码:
5-9
栏目:
临床研究
出版日期:
2024-12-20

文章信息/Info

Title:
Expression of CHI3L1 in failed autologous arteriovenous fistula of patients with end-stage renal disease and its mechanism of action
文章编号:
1002-2600(2024)08-0005-05
作者:
李露1; 张令歌1; 张乔娜1; 王红茹1; 衣利2
1 西安医学院第一附属医院肾内科,西安 710077; 2 陕西省西安市长安医院肾内科,西安 710016
Author(s):
LI Lu1; ZHANG Lingge1; ZHANG Qiaona; WANG Hongru1; YI Li2
1 Department of Nephrology, the First Affiliated Hospital, Xi'an Medical University, Xi'an,Shanxi 710077, China; 2 Department of Nephrology, Chang'an Hospital, Xi'an,Shanxi 710016, China
关键词:
肾脏替代治疗;  血液透析;  自体动静脉内瘘;  壳多糖酶-3样蛋白1
Keywords:
renal replacement therapy;  hemodialysis;  autologous arteriovenous fistula;  chitinase-3-like protein 1
分类号:
R692.5
DOI:
10.20148/j.fmj.2024.08.002
文献标志码:
B
摘要:
目的 探讨壳多糖酶-3样蛋白1(CHI3L1)在终末期肾病患者自体动静脉内瘘(AVF)组织中的表达,分析其在AVF失功中的潜在作用机制。方法 收集了9例因终末期肾病行前臂AVF成形术,或因血管瘘狭窄致AVF失功而行重建术患者的术中血管组织,采用免疫组织化学法检测局部组织中CHI3L1、基质金属蛋白酶9(MMP-9)、基质金属蛋白酶2(MMP-2)和血管内皮生长因子-A(VEGF-A)的表达情况。结果 因狭窄导致的失功AVF组织表现为腔内血栓、胶原纤维破裂、纤维结缔组织增生、管壁增厚及新生血管形成、中膜层的散在炎症细胞浸润及局部CHI3L1表达量增高,其中,初次建立AVF组CHI3L1阳性面积率中位数为0.42,因血管瘘狭窄致AVF失功行AVF重建术组CHI3L1阳性面积率中位数0.64,差异有统计学意义(P<0.05); AVF重建组MMP-2和VEGF-A的表达水平升高,且CHI3L1与MMP-2、MMP-9及VEGF-A表达水平呈正相关(r=0.902 2,P<0.05; r=0.932 3,P<0.001; r=0.833 5,P<0.05)。结论 血管组织中CHI3L1的表达可能在AVF失功中起重要作用。MMP-9、MMP-2和VEGF-A可能参与了CHI3L1导致的血管狭窄。
Abstract:
Objective To investigate the expression of CHI3L1 in autologous arteriovenous fistula(AVF)in patients with end-stage renal disease and to analyze the potential mechanism of CHI3L1 in AVF failure.Methods Immunoreactivity of CHI3L1, matrix metalloproteinase 9(MMP-9), matrix metalloproteinase 2(MMP-2)and vascular endothelial growth factor-A(VEGF-A)were detected in vascular tissues collected from nine patients who underwent AVF surgery.Due to significant stenosis, six of the nine patients received arteriovenous fistula reconstruction.Results The failure of AVF due to stenosis was characterized by intracaval thrombosis, collagen fiber rupture, fibrous connective tissue hyperplasia, vessel wall thickening and neovascularization, scattered inflammatory cell infiltration and increased CHI3L1 expression.The median CHI3L1 positive area rate in the initial AVF group was 0.42, and was 0.64 in the AVF reconstruction group due to vascular fistula stenosis. The difference was statistically significant(P<0.05).The expression levels of MMP-2 and VEGF-A increased in the AVF reconstruction group.The expression of CHI3L1 was positively correlated with MMP-2, MMP-9 and VEGF-A(r=0.902 2, P<0.05; r=0.9323, P<0.001; r=0.8335, P<0.05).Conclusion CHI3L1 expression in vascular tissues possibly plays an important role in AVF failure.MMP-2 and VEGF-A may participate in venous stenosis with CHI3L1.

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备注/Memo

备注/Memo:
基金项目:陕西省西安市2022年科技计划项目(22YXYJ0147)
通信作者:衣 利,Email:18821776176@163.com
更新日期/Last Update: 2024-12-20