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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:

46

期数:

2024年04期

页码:

6-9

栏目:

临床研究

出版日期:

2024-08-15

文章信息/Info

Title:

Analysis of fluorescence ROSE in cytological diagnosis of lung malignant tumors

文章编号:

1002-2600(2024)04-0006-04

作者:

刘加夫; 张宏英; 陈晓红; 林清华; 陈友轩; 吴联平; 林玉琼; 黄小红

福建省福州肺科医院,福州 350008

Author(s):

LIU Jiafu; ZHANG Hongying; CHEN Xiaohong; LIN Qinghua; CHEN Youxuan; WU Lianping; LIN Yuqiong; HUANG Xiaohong

Fuzhou Pulmonary Hospital,Fuzhou,Fujian 350008,China

关键词:

荧光细胞学现场快速评估;  恶性肿瘤;  CT引导经皮肺活检;  细胞学

Keywords:

fluorescence cytology rapid on-site evaluation;  malignant tumor;  CT-guided percutaneous lung biopsy;  cytology

分类号:

R734

DOI:

10.20148/j.fmj.2024.04.002

文献标志码:

B

摘要:

目的 探讨在CT引导经皮肺活检(PTLB)联合荧光细胞学现场快速评估(F-rose)在肺恶性肿瘤细胞学诊断中的应用价值。方法 收集在我院进行的微创CT引导下经皮肺穿刺活检的住院患者120例。在CT引导定位下对病变区域细针穿刺取材,将穿刺针残留液涂至两张载玻片上,并分别用另两张清洁载玻片叠放、轻压,反向匀速拉开,制成4张涂片,迅速放置于95%酒精中固定后取出干燥。通过荧光ROSE染色和ROSE染色来评估两种染色方法在肺部占位性病变中的差异性。根据组织病理学结果明确病理诊断筛选出94例患者,其中确诊恶性肿瘤66例,非肿瘤性病变28例。患者以真菌和抗酸分枝杆菌阳性为非肿瘤性病变患者。以病理组织学结果为金标准,分析两种方法对肺恶性肿瘤细胞学诊断的灵敏度及特异度,并评估两种方法对肺恶性肿瘤的细胞学诊断价值差异性。结果 荧光ROSE和ROSE组的总确诊率分别为60.64%和48.94%(P<0.05); 诊断肺部恶性肿瘤的灵敏度两组均为68.18%(P>0.05); 荧光ROSE和ROSE组的特异度分别为42.86%、3.57%(P<0.05)。结论 荧光ROSE联合PTLB可提高肺部占位性病变的确诊率,尤其可提高肺部恶性肿瘤细胞学诊断的特异度,在肺部占位性病变细胞学诊断中具有一定临床应用价值。

Abstract:

Objective To explore the clinical application of CT-guided percutaneous lung biopsy(PTLB)combined with fluorescence cytology rapid on-site evaluation(F-rose)in the cytological diagnosis of lung malignant tumors. Methods One hundred and twenty hospitalized patients who underwent minimally invasive CT-guided percutaneous lung puncture biopsy in our hospital were collected. Under CT-guided localization of the lesion area fine-needle puncture sampling,the puncture needle residual fluid was applied to two slides,and respectively,the other two clean slides were stacked,lightly pressed,and pulled apart at a uniform speed in the reverse direction to make four smears,which were quickly placed in 95% alcohol for fixation and then removed for drying. Differences between the two staining methods in lung occupying lesions were evaluated by fluorescent ROSE staining and ROSE staining. Ninety-four patients were screened for definitive pathological diagnosis based on histopathological findings,of which 66 were confirmed malignant tumors and 28 were non-neoplastic lesions.Patients with fungal and Mycobacterium antacidum-positive is non-neoplastic lesions. The sensitivity and specificity of the two methods for the cytological diagnosis of lung malignant tumors were analyzed using the pathohistological results as the gold standard,and the differences in the cytological diagnostic value of the two methods for lung malignant tumors were evaluated. Results The overall confirmed diagnosis rates of fluorescent ROSE and ROSE groups were 60.64% and 48.94% respectively(P<0.05); the sensitivity of diagnosing lung malignant tumors was 68.18% in both groups(P>0.05); and the specificity of the fluorescent ROSE and ROSE groups were 42.86% and 3.57%(P<0.05),respectively. Conclusion Fluorescence ROSE combined with PTLB can improve the confirmation rate of lung occupying lesions,especially the specificity of cytological diagnosis of lung malignant tumors,which has certain clinical application value in the cytological diagnosis of lung occupying lesions.

备注/Memo

备注/Memo:

基金项目:福州市科技局市级科技计划项目(2021-S-255)

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更新日期/Last Update: 2024-08-15