[1]赵振华,潘楚锥,雷丽平,等.血浆外泌体中铁蛋白和转铁蛋白受体水平与帕金森病相关性研究[J].福建医药杂志,2023,45(03):1-4.
　ZHAO Zhenhua,PAN Chuzhui,LEI Liping,et al.The relationship between ferritin and transferrin receptor levels in plasma exosomes and Parkinson's disease[J].FUJIAN MEDICAL JOURNAL,2023,45(03):1-4.
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血浆外泌体中铁蛋白和转铁蛋白受体水平与帕金森病相关性研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期数:
2023年03期
页码:
1-4
栏目:
临床研究
出版日期:
2023-06-15

文章信息/Info

Title:
The relationship between ferritin and transferrin receptor levels in plasma exosomes and Parkinson's disease
文章编号:
1002-2600(2023)03-0001-04
作者:
赵振华; 潘楚锥; 雷丽平; 汪银洲1
福建医科大学省立临床医学院 福建省立医院神经内科(福州 350001)
Author(s):
ZHAO Zhenhua;  PAN Chuzhui;  LEI Liping;  WANG Yinzhou
Department of Neurology, Fujian Provincial Hospital, Provincial Clinical College of Fujian Medical University, Fuzhou, Fujian 350001, China
关键词:
帕金森病;  外泌体;  铁蛋白;  转铁蛋白受体
Keywords:
Parkinson's disease;  exosomes;  ferritin;  transferrin receptor
分类号:
R713
文献标志码:
B
摘要:
目的 探讨血浆外泌体中铁蛋白和转铁蛋白水平与帕金森病之间的关系。方法 收集2019年10月至2021年10月就诊于福建省立医院的60例PD患者,同时纳入60例健康对照组,抽取两组受试者血浆,提取血浆中的外泌体,采用ELISA方法对PD患者和对照组的血浆外泌体中的铁蛋白和转铁蛋白受体进行定量分析,分析以上指标与帕金森的病程及Hoehn-Yahr(H-Y)分级的相关性。结果 PD患者血浆外泌体中铁蛋白水平较对照组明显升高[(246.87±139.61)ng/μg vs.(447.97±222.85)ng/μg,P<0.01],血浆外泌体中的转铁蛋白受体水平与对照组之间差异无统计学意义[(1 217.18±540.54)ng/μg vs.(1 236.32±389.26)ng/μg, P=0.824]。PD患者血浆外泌体中铁蛋白及转铁蛋白受体水平与PD患者的H-Y分级无明显相关性(P分别为0.966和0.453)。PD患者的病程与血浆外泌体中的铁蛋白或TfR水平的相关性分析,差异均无统计学意义(P分别为0.263或0.414)。结论 外周铁代谢紊乱可能参与了PD发生,但与疾病进展关系尚不明确,外泌体中铁蛋白可能成为帕金森病的外周血生物标志物。
Abstract:
Objective To investigate the relationship between the levels of ferritin and transferrin in plasma exosomes and Parkinson's disease.Methods A total of 60 patients with PD in Fujian Provincial Hospital from October 2019 to October 2021 were collected, and 60 healthy controls were enrolled at the same time. The plasma of the two groups was extracted and the exosomes were extracted. The ferritin and transferrin receptors in the plasma exosomes of PD patients and the control group were quantitatively analyzed by ELISA.The correlation between the above indexes,the course of Parkinson's disease and the H-Y stage was analyzed.Results Compared with the control group, the level of ferritin in the plasma exosomes of PD patients was significantly higher [(246.87±139.61)ng/μg vs.(447.97±222.85)ng/μg, P<0.01], and there was no significant difference in the level of transferrin receptor between the two groups [(1 217.18±540.54)ng/μg vs.(1 236.32±389.26)ng/μg, P=0.824].There was no significant correlation between the level of ferritin and transferrin receptor in the plasma exosomes and the course of disease or H-Y stage of PD patients(P=0.966 and 0.263, respectively).There was no significant correlation between the duration of PD and the levels of ferritin or TfR in plasma exosomes(P=0.263 and 0.414,respectively).Conclusion The disorder of peripheral iron metabolism may be involved in the occurrence of PD, but the relationship between ferritin and disease progression is not clear. Ferritin in plasma exosomes may be a biomarker of Parkinson's disease.

参考文献/References:

[1] Berardelli A,Wenning G K, Antonini A, et al.EFNS/MDS-ES/ENS [corrected] recommendations for the diagnosis of Parkinson's disease[J]. Eur J Neurol,2013,20(1):16-34.
[2] Farotti L, Paciotti S, Tasegian A, et al. Discovery,validation and optimization of cerebrospinal fluid biomarkers for use in Parkinson's disease[J].Expert Rev Mol Diagn,2017,17(8):771-780.
[3] Mehta S H, Adler C H. Advances in biomarker research in Parkinson's disease[J].Curr Neurol Neurosci Rep,2016,16(1):7.
[4] Zhao Z H,Chen Z T,Zhou R L,et al.Increased DJ-1 and α-synuclein in plasma neural-derived exosomes as potential markers for Parkinson's Disease[J].Front Aging Neurosci,2019,10:438.
[5] Postuma Ronald B, Berg D, Stern M,et al.MDS clinical diagnostic criteria for Parkinson's disease[J].Movement Disor-ders,2015,30(12):1591-1599.
[6] Youdim M B, Riederer P. The role of iron in senescence of dopaminergic neurons in Parkinson's disease [J]. J Neural Transm suppl, 1993, 40:57-67.
[7] Gutteridge J M.Hydroxyl radicals,iron,oxidative stress,and neurodegeneration [J].Ann N Y Acad sci,1994,738:201-213.
[8] Sian-Hülsmann J, Mandel S, Youdim-Moussa B H,et al. The relevance of iron in the pathogenesis of Parkinson's disease [J].J Neurochem, 2011,118(6):939-957.
[9] Griffiths P D, Dobson B R, Jones G R, et al. Iron in the basal ganglia in Parkinson's disease. An in vitro study using extended x.ray absorption fine structure and cryo.electron microscopy [J]. Brain, 1999, 122(Pt 4):667-673.
[10] An H, Zeng X, Niu T, et al. Quantifying iron deposition within the substantia nigra of Parkinson's disease by quantitative susceptibility mapping [J]. J Neurol sci,2018, 386:46,52.
[11] Dicson D W, Lin W, Liu W K. Multiple system atrophy: a sporadic synucleinopathy[J]. Brain Pathol,1999,9(4):721-732.
[12] Galvin J E, Giasson B, Hurtig H I, et al. Neurodegeneration with brain iron accumulation,type 1 is characterized by alpha-,beta-, and gamma-synuclein neuropathology [J]. Am J Pathol, 2000, 157(2): 361-368.
[13] Truman-Rosentsvit M, Berenbaum D, Spektor L,et al. Ferritin is secreted via 2 distinct nonclassical vesicular pathways[J].Blood, 2018, 131(3): 342-352.
[14] Goetzl E J,Boxer A,Schwartz J B,et al.Altered lysosomal proteins in neural-derived plasma exosomes in preclinical Alzheimer disease[J].Neurology,2015,85(1):40-47.
[15] Jiménez-Jiménez F J,Alonso-Navarro H, García-Martín E,et al.Biological fluid levels of iron and iron-related proteins in Parkinson's disease:Review and meta-analysis[J].Eur J Neurol,2021,28(3):1041-1055.
[16] Wei Z, Li X, Li X,et al.Oxidative stress in Parkinson's disease: a systematic review and meta-analysis[J].Front Mol Neurosci,2018,11:236.
[17] Wallace D F, Summerville L, Crampton E M,et al.Combined deletion of Hfe and transferrin receptor 2 in mice leads to marked dysregulation of hepcidin and iron overload[J]. Hepatology,2009,50(6):1992-2000.
[18] Wallace D F, Summerville L, Subramaniam V N. Targeted disruption of the hepatic transferrin receptor 2 gene in mice leads to iron overload[J]. Gastroenterology,2007,132(1):301-310.
[19] Fan K, Jia X, Zhou M, et al. Ferritin Nanocarrier Traverses the Blood Brain Barrier and Kills Glioma[J].Acs Nano,2018, 12(5):4105-4115.
[20] Jiang L, Wang J, Wang K, et al. RNF217 regulates iron homeostasis throμgh its E3 ubiquitin ligase activity by modulating ferroportin degradation[J]. Blood, 2021, 138(8): 689-705.
[21] 杨岚婷,徐涛,杨福情,等.转铁蛋白受体对铁过载所致大鼠心肌细胞铁死亡的作用及其机制[J].解放军医学杂志,2022,47(8):781-788.
[22] Cabrera-Valdivia F, Jiménez-Jiménez F J, Molina J A, et al. Peripheral iron metabolism in patients with Parkinson's disease[J].J Neurol Sci,1994,125(1):82-86.
[23] Matak P, Matak A, Moustafa S, et al. Disrupted iron homeostasis causes dopaminergic neurodegeneration in mice[J]. Proc Natl Acad Sci USA,2016,113(13):3428-3435.
[24] Usman W M, Pham T C, Kwok Y Y, et al. Efficient RNA drug delivery using red blood cell extracellular vesicles[J].Nat Commun,2018,9(1):2359.

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金项目(2019J01502)
1 通信作者, Email:wphd@163.com
更新日期/Last Update: 2023-06-15