[1]林燕玲,叶静,黄淑玲,等.血清VEGF、BDNF水平与老年糖尿病大鼠认知功能障碍相关性研究[J].福建医药杂志,2022,44(02):123-125.
　LIN Yanling,YE Jing,HUANG Shuling,et al.The correlation between serum levels of VEGF and BDNF and cognitive dysfunction in elderly diabetic rats[J].FUJIAN MEDICAL JOURNAL,2022,44(02):123-125.
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血清VEGF、BDNF水平与老年糖尿病大鼠认知功能障碍相关性研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期数:
2022年02期
页码:
123-125
栏目:
基础研究
出版日期:
2022-04-15

文章信息/Info

Title:
The correlation between serum levels of VEGF and BDNF and cognitive dysfunction in elderly diabetic rats
文章编号:
1002-2600(2022)02-0123-03
作者:
林燕玲; 叶静; 黄淑玲; 刘辉阳; 张伟生; 涂文劭1
福建医科大学临床医学部(福州 350108)
Author(s):
LIN Yanling;  YE Jing;  HUANG Shuling;  LIU Huiyang;  ZHANG Weisheng;  TU Wenshao
Department of Clinical Medicine, Fujian Medical University, Fuzhou, Fujian 350108, China
关键词:
老年;  糖尿病;  认知功能;  VEGF;  BDNF
Keywords:
aged;  diabetic;  cognitive function;  VEGF;  BDNF
分类号:
R587.1
文献标志码:
B
摘要:
目的 探讨血清血管内皮生长因子(VEGF)、脑源性神经营养因子(BDNF)表达水平与老年糖尿病(DM)大鼠认知功能障碍相关性,为老年DM认知功能障碍患者早期诊断和治疗提供参考依据。方法 将40只18～20月龄清洁级老年雄性SD大鼠随机分为两组:对照组(C组)和糖尿病组(T组)。C组(n=10):为老年非DM大鼠; T组(n=30):采用高糖高脂饲料喂养+腹腔内注射链脲佐菌素方法建立老年DM大鼠模型,根据成模后高血糖持续时间长短将T组进一步随机分为3个亚组:T1(1周)、T2(3周)、T3(6周),每组10只。采用Morris水迷宫测试各组大鼠认知水平,酶联免疫吸附法测定各组大鼠血清VEGF、BDNF表达水平,应用Pearson相关分析法分析血清VEGF、BDNF表达水平与老年DM大鼠Morris水迷宫测试指标的关系。结果 老年糖尿病大鼠血清VEGF、BDNF、120 s内目标象限停留时间和穿越原平台位置的次数随着高血糖持续时间的延长而降低,而潜伏期随着高血糖持续时间的延长而延长(P<0.05)。经Pearson相关分析可知,血清VEGF、BDNF表达水平与潜伏期呈负相关,而与120 s内目标象限停留时间和穿越原平台位置的次数呈正相关(P<0.05)。 结论 血清VEGF、BDNF表达降低水平与老年DM大鼠认知功能障碍严重程度正相关,临床上检测血清VEGF、BDNF表达水平对早期诊断和预防老年DM认知功能障碍发生有重要的参考意义。
Abstract:
Objective To investigate the correlation between serum levels of VEGF and BDNF and cognitive dysfunction in elderly diabetic rats, so as to provide reference for early diagnosis and treatment of elderly DM patients with cognitive dysfunction.Methods Forty 18to 20-months-old clean male SD rats were randomly divided into two groups as control group(group C)and diabetic group(group T).Group C(n=10): Aged rats without diabetes; Group T(n=30): The aged DM rat model was established by feeding a high sugar and fat diet to the rats, combined with the intraperitoneal injection of streptozotocin, according to the duration of hyperglycemia after modeling, group T was further randomly divided into three subgroups: T1(1 week), T2(3 weeks), and T3(6 weeks), with 10 rats in each subgroup.Morris water maze was used to test the cognitive level, enzyme-linked immunosorbent assay was used to determine the serum levels of VEGF and BDNF.Pearson correlation was used to analyze the relationship between the serum levels of VEGF and BDNF and Morris water maze test parameters in elderly diabetic rats.Results The serum levels of VEGF and BDNF, the residence time of target quadrant and the times of crossing the original platform within 120 s decreased with the extension of hyperglycemia duration, while the latency prolonged with the extension of hyperglycemia duration(P<0.05).Pearson correlation analysis showed that the serum levels of VEGF and BDNF were negatively correlated with the latency, but positively correlated with the residence time in the target quadrant and the times of crossing the original platform within 120 s(P<0.05).Conclusion The decreased expression of serum VEGF and BDNF are positively correlated with the severity of cognitive dysfunction in elderly DM rats.Clinically, the detection of the expression levels of serum VEGF and BDNF has important reference significance for early diagnosis and prevention of cognitive impairment in elderly DM patients.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福建医科大学大学生创新创业训练计划资助项目(C20039)
1 通信作者,福建医科大学省立临床医学院 福建省立医院麻醉科(福州 350001),Email:648065953@qq.com
更新日期/Last Update: 2022-04-15