[1]陈剑明,陈国平.UCNPs@mSiO2-PEG纳米材料的构建及其细胞靶性研究[J].福建医药杂志,2024,46(06):74-77.[doi:10.20148/j.fmj.2024.06.023]
　CHEN Jianming,CHEN Guoping.Construction of UCNPs@mSiO2-PEG nanomaterials and cellular targeting ability[J].FUJIAN MEDICAL JOURNAL,2024,46(06):74-77.[doi:10.20148/j.fmj.2024.06.023]
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UCNPs@mSiO2-PEG纳米材料的构建及其细胞靶性研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年06期
页码:
74-77
栏目:
基础研究
出版日期:
2024-11-15

文章信息/Info

Title:
Construction of UCNPs@mSiO2-PEG nanomaterials and cellular targeting ability
文章编号:
1002-2600(2024)06-0074-04
作者:
陈剑明; 陈国平
福建省福州市第二总医院,福州 350007
Author(s):
CHEN Jianming;  CHEN Guoping
Fuzhou Second General Hospital, Fuzhou, Fujian 350007, China
关键词:
上转换材料;  纳米粒子;  结肠癌
Keywords:
upconversion materials;  nanoparticles;  colon cancer
分类号:
R73
DOI:
10.20148/j.fmj.2024.06.023
文献标志码:
B
摘要:
目的 制备UCNPs@mSiO2-PEG纳米材料并考察其靶向结肠癌CT26细胞的能力。 方法 采用介孔二氧化硅包覆在上转换材料(UCNPs)来制备核-壳结构纳米粒子(UCNPs@mSiO2),在其表面进行聚乙二醇(PEG)改性,通过X射线衍射(XRD)和透射电子显微镜(TEM)对构建的UCNPs@mSiO2-PEG纳米材料进行表征,并通过激光共聚焦拍照观察其靶向结肠癌CT26细胞的能力。结果 UCNPs@mSiO2-PEG在2θ～22°处出现无定形SiO2特征衍射峰; UCNPs@mSiO2-PEG纳米粒子呈单个分散圆球形,SiO2薄膜层覆盖在UCNPs纳米粒子的表面,PEG改性层进一步覆盖在SiO2薄膜层上; 发出绿色荧光的UCNPs@mSiO2-PEG载体围绕在结肠癌CT26细胞周围。结论 成功构建核-壳结构纳米粒子UCNPs@mSiO2并进行了表面改性,改性后的UCNPs@mSiO2-PEG纳米粒子能有效靶向结肠癌CT26细胞。
Abstract:
Objective To prepare UCNPs@mSiO2-PEG nanomaterials and investigate their ability to target colon cancer CT26 cells. Methods Mesoporous silica was coated on upconversion materials to prepare coreshell structured nanoparticles(UCNPs@mSiO2). Polyethylene glycol(PEG)modification was carried out on its surface.The constructed UCNPs@mSiO2-PEG nanomaterials were characterized by X-ray diffraction(XRD)and transmission electron microscopy(TEM). The ability to target colon cancer CT26 cells was observed by laser confocal photography. Results UCNPs@mSiO2-PEG showed an amorphous SiO2 characteristic diffraction peak at 2θ～22°. UCNPs@mSiO2-PEG nanoparticles were in a single dispersed spherical shape. The SiO2 film layer covered the surface of UCNPs nanoparticles, and the PEG modification layer further covered the SiO2 film layer. The UCNPs@mSiO2-PEG carrier emitting green fluorescence surrounded the colon cancer CT26 cells. Conclusion The coreshell structured nanoparticles UCNPs@mSiO2 were successfully constructed and surface-modified. The modified UCNPs@mSiO2-PEG nanoparticles can effectively target colon cancer CT26 cells.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金资助项目(2022J011314)
通信作者:陈国平,Email:chenguoping_fj@126.com
更新日期/Last Update: 2024-11-15