[1]张晓芬,吴世忠,魏德.海马神经连接蛋白-2参与三叉神经痛大鼠模型焦虑样行为[J].福建医药杂志,2024,46(06):71-74.[doi:10.20148/j.fmj.2024.06.022]
　ZHANG Xiaofen,WU Shizhong,WEI De.Hippocampal Neuroligin-2 is involved in anxiety-like behavior in a rat model of trigeminal neuralgia[J].FUJIAN MEDICAL JOURNAL,2024,46(06):71-74.[doi:10.20148/j.fmj.2024.06.022]
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海马神经连接蛋白-2参与三叉神经痛大鼠模型焦虑样行为()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年06期
页码:
71-74
栏目:
基础研究
出版日期:
2024-11-15

文章信息/Info

Title:
Hippocampal Neuroligin-2 is involved in anxiety-like behavior in a rat model of trigeminal neuralgia
文章编号:
1002-2600(2024)06-0071-04
作者:
张晓芬1; 吴世忠2; 魏德2
1 厦门医学院基础医学部人体解剖学与组织胚胎学教研室,厦门 361000; 2 福建医科大学省立临床医学院神经外科,福州 350001
Author(s):
ZHANG Xiaofen1;  WU Shizhong2;  WEI De2
1 Department of Human Anatomy and Embryology, Basic Medical Science, Xiamen Medical College, Xiamen, Fujian 361000, China; 2 Department of Neurosurgery, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 35
关键词:
三叉神经痛;  腹侧海马;  痛焦虑;  星形胶质细胞;  神经连接蛋白
Keywords:
trigeminal neuralgia;  ventral hippocampus;  anxiety-like pain;  astrocytes;  neuroligin-2
分类号:
R3; R749.7+2
DOI:
10.20148/j.fmj.2024.06.022
文献标志码:
A
摘要:
目的 研究在三叉神经痛(TN)大鼠腹侧海马的神经连接蛋白-2(Neuroligin-2)表达变化及其在星形胶质细胞的表达情况。方法 通过大鼠三叉神经根(TREZ)慢性压迫损伤诱导建立TN动物模型,应用纤毛机械刺激针测大鼠口面部机械痛阈值、旷场实验、高架十字迷宫测定动物焦虑水平、自主活动情况。术后21 d取材,采用免疫荧光染色以及蛋白质印迹检测腹侧海马Neuroligin-2的表达变化。结果 经TREZ慢性压迫损伤后,TN大鼠具有焦虑样行为表现,腹侧海马的A1型星形胶质细胞被大量激活,并诱导其来源的Neuroligin-2表达增多。结论 大鼠TREZ慢性压迫激活可诱导星形胶质细胞来源的Neuroligin-2表达增多,可能通过改变海马神经元兴奋性和抑制性突触比例,参与焦虑样行为的产生。
Abstract:
Objective To investigate the expression of Neuroligin-2 and its expression in astrocytes in the ventral hippocampus of trigeminal neuralgia(TN)rats. Methods Trigeminal nerve root entry zone(TREZ)chronic compression injury was used to induce TN animal model. Orofacial mechanical pain threshold was measured by von Frey, open field test and elevated plus maze were used to measure autonomic activity. Immunofluorescence staining and Western blot were used to detect the expression of Neuroligin-2 in the ventral hippocampus at 21 days after operation. Results TN rats showed anxiety-like behavior after chronic compression injury with TREZ. A1 astrocytes in the ventral hippocampus were heavily activated and the expression of astrocyte-derived Neuroligin-2 was induced. Conclusion Chronic compression and activation of TREZ in rats can induce increased expression of astrocyte-derived Neuroligin-2, which may be involved in the generation of anxiety-like behavior by changing the ratio of excitatory and inhibitory synapses of hippocampal neurons.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福建省卫生教育联合攻关计划项目(2019-WJ-26)
通信作者:魏 德,Email: weidele@fjmu.edu.cn
更新日期/Last Update: 2024-11-15