[1]廖常希,蔡清河,江小杰,等.PIEZO2通过改变细胞功能促进胰腺癌细胞的发生发展[J].福建医药杂志,2024,46(06):21-24.[doi:10.20148/j.fmj.2024.06.006]
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PIEZO2通过改变细胞功能促进胰腺癌细胞的发生发展()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年06期
页码:
21-24
栏目:
临床研究
出版日期:
2024-11-15

文章信息/Info

文章编号:
1002-2600(2024)06-0021-04
作者:
廖常希蔡清河江小杰张如婧
莆田学院附属医院,莆田 351100
关键词:
胰腺癌细胞 PIEZO2 转录组 Panc-1 细胞功能
分类号:
R73
DOI:
10.20148/j.fmj.2024.06.006
文献标志码:
A
摘要:
目的 探讨压电型机械敏感离子通道元件2(piezoelectric type mechanosensitive ion channel component 2,PIEZO2)对胰腺癌细胞发生发展的影响。方法 采用免疫组织化学技术检测胰腺癌与癌旁组织的PIEZO2阳性率; 将PIEZO2干扰片段转染到胰腺癌细胞Panc-1中,通过实时荧光定量PCR(qPCR)和蛋白免疫印迹(WB)检测PIEZO2的转录水平及蛋白水平。以细胞计数试剂盒-8(Cell Counting Kit-8,CCK8)检测细胞增殖情况,transwell法检测细胞的迁移侵袭能力。通过Illumina HiSeqTMX Ten进行cDNA文库构建并测序,分析干扰PIEZO2后Panc-1细胞内差异基因并通过GO富集分析参与基因富集的相关通路。结果 胰腺癌组织中PIEZO2表达水平高于癌旁组织,且高PIEZO2表达的胰腺癌患者总生存率较低。敲减PIEZO2基因后,qPCR和WB结果显示PIEZO2的表达量较对照组减低,差异具有统计学意义。敲减组的增殖、迁移、侵袭能力均低于对照组,且差异具有统计学意义。对两组进行转录组分析发现,两组分别获得15 521,15 325个基因,与敲减组相比,对照组有3 411个差异表达基因(differentially expressed genes,DEGs)上调,2 830个DEGs下调,GO富集分析发现DEGs基因主要富集于细胞内解剖结构、细胞连接和细胞外基质。结论 敲除PIEZO2可抑制细胞的增殖、迁移和侵袭,PIEZO2通过调控细胞解剖结构、细胞连接和细胞外基质参与胰腺癌的发生发展,靶向PIEZO2将是胰腺癌新的治疗策略。

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备注/Memo

备注/Memo:
基金项目:福建省卫生健康科技计划项目(2020QNB051)
通信作者:张如婧,Email:Zhangrujing26@163.com
更新日期/Last Update: 2024-11-15