[1]姚义,张明基.骨髓间充质干细胞对重症急性胰腺炎大鼠相关肺损伤的实验研究[J].福建医药杂志,2024,46(01):120-124181.[doi:10.20148/j.fmj.2024.01.033]
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骨髓间充质干细胞对重症急性胰腺炎大鼠相关肺损伤的实验研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年01期
页码:
120-124181
栏目:
基础研究
出版日期:
2024-02-15

文章信息/Info

文章编号:
1002-2600(2024)01-0120-06
作者:
姚义张明基
福建医科大学肿瘤临床医学院 福建省肿瘤医院肝胆胰外科,福州 350014
关键词:
重症急性胰腺炎肺损伤骨髓间充质干细胞通透性紧密连接蛋白
分类号:
R657.5
DOI:
10.20148/j.fmj.2024.01.033
文献标志码:
B
摘要:
目的探讨骨髓间充质干细胞(BMMSCs)对重症急性胰腺炎(SAP)大鼠肺损伤中ZO-1、Occludin表达的影响。方法将105只雄性SD大鼠随机分成假手术组(SO组)、SAP组和 BMMSCs干预组;后两组再按处死时间不同分为6 h、12 h和24 h组,观察各组间肺的病理变化及肺毛细血管通透性改变情况;通过Western blot、RT-PCR检测各组间ZO-1、Occludin蛋白和mRNA的改变情况。结果与SO组相比,SAP组各时间点肺、胰腺的病理改变明显,肺毛细血管的通透性明显增加(P<0.05),肺湿干比值增加(P<0.05),肺ZO-1、Occludin蛋白和mRNA表达明显降低(P<0.05);与SAP组比较,BMMSCs干预组各时间点肺、胰腺的病理改变减轻,肺毛细血管通透性改变减轻,肺组织湿干比值降低,肺ZO-1、Occludin蛋白和mRNA表达升高,12 h和24 h时间点表现的更为明显(P<0.05)。结论 BMMSCs可使肺中ZO-1、Occludin的表达增加,这可能在减轻SAP相关肺损伤和改善肺毛细血管通透性方面发挥重要作用。

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备注/Memo

备注/Memo:
通信作者:张明基,Email:union3328@163.com
更新日期/Last Update: 2024-02-15