[1]林洁,廖心成,钱彬.和厚朴酚抑制IDO1表达改善老年小鼠术后认知功能障碍的实验研究[J].福建医药杂志,2023,45(06):111-115.
　LIN Jie,LIAO Xincheng,QIAN Bin.Honokiol improves postoperative cognitive dysfunction by inhibiting indoleamine 2,3-dioxygenase 1 in elderly mice[J].FUJIAN MEDICAL JOURNAL,2023,45(06):111-115.
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和厚朴酚抑制IDO1表达改善老年小鼠术后认知功能障碍的实验研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期数:
2023年06期
页码:
111-115
栏目:
基础研究
出版日期:
2023-12-15

文章信息/Info

Title:
Honokiol improves postoperative cognitive dysfunction by inhibiting indoleamine 2,3-dioxygenase 1 in elderly mice
文章编号:
1002-2600(2023)06-0111-05
作者:
林洁; 廖心成1; 钱彬2
福建中医药大学附属人民医院麻醉科(福州 350004)
Author(s):
LIN Jie;  LIAO Xincheng;  QIAN Bin
Department of Anesthesiology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, China
关键词:
认知功能障碍;  和厚朴酚;  色氨酸代谢;  吲哚胺2; 3双加氧酶1(IDO1)
Keywords:
cognitive dysfunction;  honokiol;  tryptophan metabolism;  indoleamine 2; 3-dioxygenase 1
分类号:
R589
文献标志码:
A
摘要:
目的 探讨和厚朴酚对术后认知功能障碍防治作用的机制。方法 将18月龄雄性C57BL/6小鼠随机分为4组:Control组(溶剂预处理7 d,不进行手术)、HNK组(10 mg/kg和厚朴酚预处理7 d,不进行手术)、Surgery组(溶剂预处理7 d,脾切除术建模)和Surgery+HNK组(10 mg/kg和厚朴酚预处理7 d,脾切除术建模)。运用Morris水迷宫评估空间记忆功能,免疫荧光染色观察海马凋亡神经元比例,检测海马Bcl-2、Bax、活化Caspase-3以及吲哚胺2,3双加氧酶1(indoleamine 2,3-dioxygenase 1, IDO1)表达水平,高效液相色谱检测色氨酸毒性代谢产物含量。结果 与Surgery组相比,Surgery+HNK组水迷宫测试期第1、3、7天目标象限停留时间和平台穿越次数增加; 术后第7天海马凋亡神经元比例降低; 术后第1天Bcl-2表达增高、Bax表达水平降低、活化Caspase-3水平降低,IDO1表达降低; 色氨酸毒性代谢物降低。结论 和厚朴酚可通过抑制IDO1表达,调控色氨酸代谢,减轻海马神经元损伤,改善小鼠术后认知功能障碍。
Abstract:
Objective To explore the mechanism by which honokiol improves postoperative cognitive dysfunction(POCD)in elderly mice.Methods The 18-month-old male C57BL/6J mice were randomly divided into four groups: control group, HNK group, surgery group and surgery+HNK group.We conducted splenectomy to establish a POCD animal model.The Morris water maze(MWM)test was performed to assess the hippocampus-related cognitive function.TdT-mediated dUTP nick end labeling(TUNEL)staining was used to assess the proportion of apoptotic neurons of the hippocampus at 24 h postope-ratively.The hippocampal levels of Bcl-2, Bax, cleaved caspase-3, and indoleamine 2,3-dioxygenase 1(IDO1)were evaluated using western blotting.The contents of tryptophan metabolites kynurenine, 3-hydroxykynurenine(3-HK), 3-hydroxyanthranilic acid(3-HAA), and quinolinic acid(QA)were also detected using high performance liquid chromatography(HPLC).Results Compared with the surgery group, the surgery+HNK group mice showed increased target quadrant residence time and platform crossing times on the 1st, 3rd, and 7th day of the MWM testing period; The proportion of apoptotic neurons of hippocampal decreased in the surgery+HNK group on postoperative day 7; Honokiol pretreatment increased the level of Bcl-2 and decreased the levels of Bax, cleaved caspase-3, and IDO1 in the hippocampus on postoperative day 1.The HPLC results showed that honokiol pretreatment also reduced the contents of kynurenine, 3-HK, 3-HAA and QA in the hippocampus on postoperative day 1.Conclusion Honokiol alleviates hippocampal neuronal damage and improves POCD in elderly mice by inhibiting IDO1 expression and regulating hippocampal tryptophan metabolism.

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备注/Memo

备注/Memo:
基金项目:福建省中青年教师教育科研项目(JAT220114); 福建省自然科学基金(2022J01847)
1 福建中医药大学附属第二人民医院麻醉科; 2 通信作者
更新日期/Last Update: 2023-12-15