[1]黄永玲.Beagle犬牙周韧带成纤维细胞和骨髓基质细胞体外成骨再生潜能的比较[J].福建医药杂志,2023,45(01):106-110.
 HUANG Yongling.Comparison of osteopenic potential of PDLFs and BMSCs in Beagle dog[J].FUJIAN MEDICAL JOURNAL,2023,45(01):106-110.
点击复制

Beagle犬牙周韧带成纤维细胞和骨髓基质细胞体外成骨再生潜能的比较()
分享到:

《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
45
期数:
2023年01期
页码:
106-110
栏目:
基础研究
出版日期:
2023-02-15

文章信息/Info

Title:
Comparison of osteopenic potential of PDLFs and BMSCs in Beagle dog
文章编号:
1002-2600(2023)01-0106-05
作者:
黄永玲
福建省口腔疾病研究重点实验室 福建省口腔生物材料工程技术研究中心 福建省高校口腔医学重点实验室 福建医科大学口腔医学院/附属口腔医院(福州 350002)
Author(s):
HUANG Yongling
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University & School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian 350002, Ch
关键词:
骨髓基质细胞 牙周韧带成纤维细胞 聚合酶链反应 碱性磷酸酶 骨保护素
Keywords:
bone marrow stromatolites cells periodontal ligament fibroblasts cells realtime PCR alkaline phosphatase osteoprotegerin
分类号:
R781
文献标志码:
A
摘要:
目的 比较Beagle犬牙周韧带成纤维细胞(periodontal ligament fibroblasts cells,PDLFs)和骨髓基质细胞(bone marrow stromal cells,BMSCs)体外成骨潜能,为筛选合适的种子细胞促进牙周组织再生提供实验依据。方法 体外通过茜素红染色、Realtime-PCR比较两种细胞碱性磷酸酶(alkaline phosphatase,ALP)及骨保护素(osteoprotegerin,OPG)的表达。结果 茜素红染色显示PDLFs成骨能力高于BMSCs; Realtime-PCR显示,PDLFs的OPG表达量高于BMSCs,差别有统计学意义(P<0.05); PDLFs的ALP表达量虽低于BMSCs,但两者之间差别无统计学意义(P>0.05)。结论 PDLFs体外成骨潜能不低于BMSCs。
Abstract:
Objective To compare the osteopenic potential between periodontal ligament fibroblasts cells(PDLFs) and bone marrow stromal cells(BMSCs)in Beagle dog, and provide foundation for selecting seed cells of periodontal tissue regeneration.Methods Alizarin red staining and realtime PCR were used to compare the expression of alkaline phosphatase(ALP)and osteoprotegerin(OPG)between PDLFs and BMSCs.Results The result of Alizarin red staining showed that the osteopenic potential of PDLFs was higher than that of BMSCs.Date of realtime PCR showed that the levels of OPG in PDLFs was higher than that in BMSCs(P<0.05); the levels of ALP in PDLFs was lower than that in BMSCs, but the result had no statistical significance(P>0.05).Conclusion The osteopenic potential of PDLFs was not lower than that of BMSCs.

参考文献/References:

[1] Houshmand B, Behnia H, Khoshzaban A, et al.Osteoblastic differentiation of human stem cells derived from bone marrow and periodontal ligament under the effect of enamel matrix derivative and transforming growth factor-beta[J].Int J Oral Maxillofac Implants, 2013, 28(6): e440-e450.
[2] Tomokiyo A, Wada N, Maeda H.Periodontal ligament stem cells: Regenerative potency in periodontium[J].Stem Cells Dev, 2019, 28(15):974-985.
[3] 张梦涵, 何智琪, 邓辉, 等.褪黑素对人牙周膜细胞成骨分化能力的影响[J].口腔医学, 2021, 41(3):198-203.
[4] 李韦瑶, 邓嘉强, 赵芳芳, 等.姜黄素对犬骨髓间充质干细胞迁移能力的影响[J].四川农业大学学报, 2022, 40(4): 24:536-542.
[5] 林敏魁, 黄永玲, 钟泉, 等.比格犬骨髓基质细胞和牙周韧带成纤维细胞体外再生组织能力的比较[J].福建医科大学学报, 2011, 45(4):242-245.
[6] Tomokiyo A, Wada N, Maeda H.Periodontal ligament stem cells: regenerative potency in periodontium.[J].Stem Cells Dev, 2019, 28(15):974-985.
[7] Hu L, Liu Y, Wang S.Stem cell-based tooth and periodontal regeneration[J].Oral Dis, 2018,24(5):696-705.
[8] Nagata M, Iwasaki K, Akazawa K, et al.Conditioned medium from periodontal ligament stem cells enhances periodontal regeneration[J].Tissue Eng Part A, 2017, 23(9-10):367-377.
[9] Iwasaki K, Akazawa K, Nagata M, et al.The fate of transplanted periodontal ligament stem cells in surgically created periodontal defects in rats[J].Int J Mol Sci, 2019, 20(1):192.
[10] 金振宇,张志宏.犬骨髓基质细胞矿化不同阶段对犬牙周膜干细胞增殖和分化的影响[J].口腔疾病防治, 2018, 26(11):699-705.
[11] Liu L, Guo S, Shi W, et al.Bone marrow mesenchymal stem cell-derived small extracellular vesicles promote periodontal regeneration[J].Tissue Eng Part A, 2021, 27(13-14):962-976.
[12] Tsumanuma Y, Iwata T, Washio K, et al.Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model[J].Biomaterials, 2011, 32(25):5819-5825.
[13] Zhou Q, Gu X, Dong J, et al.The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environmen[J].Artif Cells Nanomed Biotechnol, 2019, 47(1):3329-3337.
[14] 王松, 王启松, 何梦娇, 等.骨髓基质细胞膜片对骨质疏松大鼠牙周组织再生的影响[J].中华骨质疏松和骨矿盐疾病杂志, 2019, 12(6):586-594.
[15] Inoue T, Osada H, Shiigai T, et al.An experimental study of osteogenesis by autografted dental pulp, periodontal ligament, and bone marrow in vivo[J].Bull Tokyo Dent Coll, 1993, 34(4):183-190.
[16] Grzesik W J, Kuzentsov S A, Uzawa K, et al.Normal human cementum-derived cells: isolation, clonal expansion, and in vitro and in vivo characterization[J].J Bone Miner Res, 1998, 13(10):1547-1554.
[17] 徐孟丹, 周咏, 皱多宏.拉布拉多犬骨髓基质干细胞与牙周干细胞裸鼠皮下成骨能力的比较研究[J].安徽医科大学学报, 2016, 51(9):1244-1247.
[18] Yan X Z,Yang F, Jansen J A, et al.Cell-Based approaches in periodontal regeneration: a systematic review and meta-analysis of periodontal defect models in animal experimental work[J].Tissue Eng Part B Rev, 2015, 21(5):411-426.
[19] Tassi S A, Sergio N Z, Misawa M Y O, et al.Efficacy of stem cells on periodontal regeneration: Systematic review of pre-clinical studies[J].J Periodontal Res, 2017, 52(5):793-812.
[20] 王娉婷, 周盼, 郑国莹,等.吡咯烷二硫氨基甲酸干预脂多糖刺激下牙周膜干细胞RANKL和OPG的表达[J].中国组织工程研究, 2018, 22(21):3365-3370.
[21] Sengottuvelan A, Balasubramanian P, Will J, et al.Bioactivation of titanium dioxide scaffolds by ALP-functionalization[J].Bioact Mater, 2017, 2(2):108-115.
[22] 黄永玲, 骆凯,李艳芬,等.犬牙周膜细胞与骨髓基质细胞组织再生能力比较的实验研究[J].福建医科大学学报, 2016,50(6):365-369.

更新日期/Last Update: 2023-02-15