[1]林鑫,陈秀兰,姚秀娟,等.自噬介导NLRP3活化对小鼠肺纤维化的影响[J].福建医药杂志,2022,44(04):126-128136.
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自噬介导NLRP3活化对小鼠肺纤维化的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期数:
2022年04期
页码:
126-128136
栏目:
基础研究
出版日期:
2022-08-15

文章信息/Info

文章编号:
1002-2600(2022)04-0126-04
作者:
林鑫; 陈秀兰1; 姚秀娟2; 谢宝松2; 3
福建中医药大学（福州 350122）
关键词:
肺纤维化; 自噬; NLRP3; Notch1; 博来霉素
分类号:
R563
文献标志码:
B
摘要:
目的 探讨肺纤维化小鼠自噬活性改变对NLRP3炎性体活化的影响。方法 将32只雄性SFP级C57BL/6小鼠随机分为对照组（A组）、实验组（B组、C组、D组），每组各8只小鼠。实验组B组、C组、D组雾化吸入剂量为35 mg/kg博来霉素（BLM），并分别在给药后第7天、第14天、第28天处死， A组雾化吸入相同容积生理盐水，并在给药后第28天处死。HE染色观察小鼠肺组织病理改变；Masson染色计算肺组织胶原纤维沉积；蛋白免疫印迹法（Western blot）检测肺组织p62、Beclin1、NLRP3、Caspase1、Notch1的表达水平。结果 HE染色结果显示，A组小鼠肺组织肺泡结构基本正常，未见明显纤维化；B组、C组、D组小鼠肺组织纤维化程度逐渐加重，可见纤维化病灶逐渐扩大，肺间质纤维组织增生。Masson染色显示，肺间质有少许蓝色染色区域。B组、C组、D组，蓝色染色区域依次增加，肺间质蓝色染色区域面积差异有统计学意义(P<0.05)。Western blot结果显示，A组p62相对表达量低于各实验组，实验组B、C、D组之间差异有统计学意义(P<0.05)；A组Beclin1表达量高于各实验组，实验组B、C、D组之间差异有统计学意义(P<0.05)；A组NLRP3、Caspase1、Notch1表达量均低于实验组，差异均有统计学意义（P<0.05）。实验组B、C、D组中NLRP3、Caspase1、Notch1之间的表达量差异均有统计学意义（P<0.05）。结论 自噬抑制和NLRP3炎性小体激活在小鼠肺纤维化中扮演重要角色；自噬活性降低，Notch1信号通路活化后导致NLRP3炎性小体激活，其可能是小鼠肺纤维化逐渐加重的机制之一。

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备注/Memo

备注/Memo:
1 福建省老年病医院；2 福建医科大学省立临床医学院呼吸与危重学科；3 通信作者
更新日期/Last Update: 2022-08-15