[1]林燕玲,叶静,黄淑玲,等.血清VEGF、BDNF水平与老年糖尿病大鼠认知功能障碍相关性研究[J].福建医药杂志,2022,44(02):123-125.
　LIN Yanling,YE Jing,HUANG Shuling,et al.The correlation between serum levels of VEGF and BDNF and cognitive dysfunction in elderly diabetic rats[J].FUJIAN MEDICAL JOURNAL,2022,44(02):123-125.
点击复制

血清VEGF、BDNF水平与老年糖尿病大鼠认知功能障碍相关性研究()
分享到：

《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
44
期数:
2022年02期
页码:
123-125
栏目:
基础研究
出版日期:
2022-04-15

文章信息/Info

Title:
The correlation between serum levels of VEGF and BDNF and cognitive dysfunction in elderly diabetic rats
文章编号:
1002-2600(2022)02-0123-03
作者:
林燕玲; 叶静; 黄淑玲; 刘辉阳; 张伟生; 涂文劭1
福建医科大学临床医学部(福州 350108)
Author(s):
LIN Yanling;  YE Jing;  HUANG Shuling;  LIU Huiyang;  ZHANG Weisheng;  TU Wenshao
Department of Clinical Medicine, Fujian Medical University, Fuzhou, Fujian 350108, China
关键词:
老年;  糖尿病;  认知功能;  VEGF;  BDNF
Keywords:
aged;  diabetic;  cognitive function;  VEGF;  BDNF
分类号:
R587.1
文献标志码:
B
摘要:
目的 探讨血清血管内皮生长因子(VEGF)、脑源性神经营养因子(BDNF)表达水平与老年糖尿病(DM)大鼠认知功能障碍相关性,为老年DM认知功能障碍患者早期诊断和治疗提供参考依据。方法 将40只18～20月龄清洁级老年雄性SD大鼠随机分为两组:对照组(C组)和糖尿病组(T组)。C组(n=10):为老年非DM大鼠; T组(n=30):采用高糖高脂饲料喂养+腹腔内注射链脲佐菌素方法建立老年DM大鼠模型,根据成模后高血糖持续时间长短将T组进一步随机分为3个亚组:T1(1周)、T2(3周)、T3(6周),每组10只。采用Morris水迷宫测试各组大鼠认知水平,酶联免疫吸附法测定各组大鼠血清VEGF、BDNF表达水平,应用Pearson相关分析法分析血清VEGF、BDNF表达水平与老年DM大鼠Morris水迷宫测试指标的关系。结果 老年糖尿病大鼠血清VEGF、BDNF、120 s内目标象限停留时间和穿越原平台位置的次数随着高血糖持续时间的延长而降低,而潜伏期随着高血糖持续时间的延长而延长(P<0.05)。经Pearson相关分析可知,血清VEGF、BDNF表达水平与潜伏期呈负相关,而与120 s内目标象限停留时间和穿越原平台位置的次数呈正相关(P<0.05)。 结论 血清VEGF、BDNF表达降低水平与老年DM大鼠认知功能障碍严重程度正相关,临床上检测血清VEGF、BDNF表达水平对早期诊断和预防老年DM认知功能障碍发生有重要的参考意义。
Abstract:
Objective To investigate the correlation between serum levels of VEGF and BDNF and cognitive dysfunction in elderly diabetic rats, so as to provide reference for early diagnosis and treatment of elderly DM patients with cognitive dysfunction.Methods Forty 18to 20-months-old clean male SD rats were randomly divided into two groups as control group(group C)and diabetic group(group T).Group C(n=10): Aged rats without diabetes; Group T(n=30): The aged DM rat model was established by feeding a high sugar and fat diet to the rats, combined with the intraperitoneal injection of streptozotocin, according to the duration of hyperglycemia after modeling, group T was further randomly divided into three subgroups: T1(1 week), T2(3 weeks), and T3(6 weeks), with 10 rats in each subgroup.Morris water maze was used to test the cognitive level, enzyme-linked immunosorbent assay was used to determine the serum levels of VEGF and BDNF.Pearson correlation was used to analyze the relationship between the serum levels of VEGF and BDNF and Morris water maze test parameters in elderly diabetic rats.Results The serum levels of VEGF and BDNF, the residence time of target quadrant and the times of crossing the original platform within 120 s decreased with the extension of hyperglycemia duration, while the latency prolonged with the extension of hyperglycemia duration(P<0.05).Pearson correlation analysis showed that the serum levels of VEGF and BDNF were negatively correlated with the latency, but positively correlated with the residence time in the target quadrant and the times of crossing the original platform within 120 s(P<0.05).Conclusion The decreased expression of serum VEGF and BDNF are positively correlated with the severity of cognitive dysfunction in elderly DM rats.Clinically, the detection of the expression levels of serum VEGF and BDNF has important reference significance for early diagnosis and prevention of cognitive impairment in elderly DM patients.

参考文献/References:

[1] Dominguez R O,Pagano M A,Marschoff E R,et al.Alzheimer disease and cognitive impairment associated with diabetes mellitus type 2:associations and a hypothesis[J].Neurologia,2014,29(9):567-572.
[2] 周雪梅,王丽香,贾文歆等.非痴呆型血管性认知障碍患者血清BDNF、GDNF水平的测定及其临床意义[J].中国实用医药,2020,15(1):93-94.
[3] 郑晓春,吴桂寿,涂文劭等.七氟烷对糖尿病相关认知功能下降老年大鼠海马谷氨酸和γ-氨基丁酸的影响[J].福建医科大学学报,2015,49(2):88-91.
[4] 汪峰,何玉,刘宓,等.糖尿病认知功能障碍大鼠模型的建立及其认知功能障碍观察[J].重庆医学,2015,44(16):2234-2236.
[5] Pietrelli A,Matkovic L,Vacotto M,et al.Aerobic exercise upregulates the BDNF-Serotonin systems and improves the cognitive function in rats[J].Neurobiol Learn Mem,2018,155:528-542.
[6] Maass A,Düzel S,Brigadski T,et al.Relationships of pe-ripheral IGF-1,VEGF and BDNF levels to exercise-related changes in memory,hippocampal perfusion and volumes in older adults[J].Neuroimage,2016,131:142-154.
[7] 张茗,纪红,严之红.老年2型糖尿病认知功能障碍患者血清胃促生长素和血管内皮生长因子水平变化及临床意义[J].中华实用诊断与治疗杂志,2019,33(12):1198-1201.
[8] Mazidi M,Rezaie P,Kengne AP,et al.VEGF,the underlying factor for metabolic syndrome; fact or fiction?[J].Diabetes Metab Syndr,2017,11(Suppl 1):S61-S64.

相似文献/References:

[1]方剑锋.三联疗法两种疗程治疗老年幽门螺杆菌阳性消化性溃疡患者疗效分析[J].福建医药杂志,2017,39(4):122.
[2]潘金兴 姜锋.老年2型糖尿病患者血清CA199水平及其与血糖的相关性[J].福建医药杂志,2017,39(5):119.
[3]李 敏 姜 锋 魏潇琪 王小易 林燕清 郑炜平.Kisspeptin对胰岛β细胞的凋亡影响及作用机制研究[J].福建医药杂志,2020,42(01):125.
　LI Min,JIANG Feng,WEI Xiaoqi,et al.Study on the effect and mechanism of kisspeptin on apoptosis of islet β cells[J].FUJIAN MEDICAL JOURNAL,2020,42(02):125.
[4]林建光,许天文.白蛋白紫杉醇与多西他赛一线治疗老年晚期肺鳞癌的疗效比较[J].福建医药杂志,2021,43(01):51.
[5]林增钰,袁 音,黄 峰,等.社区老年人空巢状态与营养不良风险的相关性研究[J].福建医药杂志,2021,43(05):19.
　LIN Zengyu,YUAN Yin,HUANG Feng,et al.Association between empty-nest status and malnutrition risk among community-dwelling elderly population[J].FUJIAN MEDICAL JOURNAL,2021,43(02):19.
[6]庄宸麟,余惠珍陈毓岱,孙嘉峰,等.控制营养状态评分与老年慢性心衰急性发作患者死亡预后的相关性研究[J].福建医药杂志,2023,45(02):16.
　ZHUANG Chenlin,YU Huizhen,CHEN Yudai,et al.Study on the correlation between controlling nutritional status score and death prognosis in elderly patients with acute episode of chronic heart failure[J].FUJIAN MEDICAL JOURNAL,2023,45(02):16.
[7]连学辉,肖红利,杨明,等.老年胸腰椎骨折60例术后炎症反应与疼痛关系分析[J].福建医药杂志,2023,45(02):25.

备注/Memo

备注/Memo:
基金项目:福建医科大学大学生创新创业训练计划资助项目(C20039)
1 通信作者,福建医科大学省立临床医学院 福建省立医院麻醉科(福州 350001),Email:648065953@qq.com
更新日期/Last Update: 2022-04-15