[1]章德昆 陈婷婷 陈 魁 王力群 龚 超 李 嵩 夏垚坤 陈敬华.一种用于microRNA-21浓度检测的荧光传感方法的建立[J].福建医药杂志,2020,42(01):132-134.
　ZHANG Dekun,CHEN Tingting,CHEN Kui,et al.Establishment of a fluorescence sensing method for microRNA-21 concentration detection[J].FUJIAN MEDICAL JOURNAL,2020,42(01):132-134.
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一种用于microRNA-21浓度检测的荧光传感方法的建立()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
42
期数:
2020年01期
页码:
132-134
栏目:
基础研究
出版日期:
2020-03-30

文章信息/Info

Title:
Establishment of a fluorescence sensing method for microRNA-21 concentration detection
文章编号:
1002-2600(2020)01-0132-03
作者:
章德昆1 陈婷婷2 陈 魁 王力群 龚 超 李 嵩 夏垚坤2 陈敬华2
福建医科大学附属福州市第一医院(福州 350009)
Author(s):
ZHANG Dekun;  CHEN Tingting;  CHEN Kui;  WANG Liqun;  GONG Chao;  LI Song;  XIA Yaokun;  CHEN Jinghua.
The Affiliated Fuzhou Municipal First Hospital of Fujian Medical University, Fuzhou,Fujian 350009, China
关键词:
聚多巴胺纳米粒子;  miRNA-21;  DNA探针;  荧光检测
Keywords:
polydopamine nanoparticles;  miRNA-21;  DNA probe;  fluorescence detection
分类号:
R917
文献标志码:
A
摘要:
目的 建立一种用于microRNA-21(miRNA-21)浓度检测的,基于聚多巴胺纳米粒子(PDANs)的荧光传感方法。方法 Ce6标记的捕获探针(Ce6-DNA)在Ca2+的介导下吸附在PDANs表面,荧光被猝灭。miRNA-21和Ce6-DNA杂交后,使其从PDANs表面解吸附,荧光恢复。利用荧光分光光度计分析靶标和Ce6-DNA反应前后荧光强度的变化,实现对miRNA-21的检测。结果 在5.0～200 nM范围内,670 nm处的荧光强度和miRNA-21的浓度呈良好的线性关系,检测限为1.80 nM。结论 基于Ce6-DNA功能化的PDANs可为miRNA-21的定量检测提供一种简单、准确、成本低、分析速度快的新方法。
Abstract:
Objective To develop a fluorescent method based on polydopamine nanoparticles(PDANs)for microRNA-21(miRNA-21)concentration detection. Methods Ce6-labeled capture probe(Ce6-DNA)was absorbed on the surface of PDANs through Ca2+ mediation, which resulted in the fluorescence of Ce6 being quenched. However, the Ce6-DNA was desorbed from PDANs when it hybridized with miRNA-21, which led to the recovery of fluorescence. This method could detect miRNA-21 quantitatively through measuring the change of fluorescence intensity using fluorescent spectrophotometer. Results There was a good linear relationship between the fluorescence intensity at 670 nm and miRNA-21 concentration from 5.0 to 200 nM. The limit of detection was 1.80 nM. Conclusion The Ce6-DNA functionalized PDANs can provide a simple, accurate, low-cost, and rapid novel method for miRNA-21 detection.

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金(2017J01226)1 Email:13809500062@139.com; 2 福建医科大学药学院
更新日期/Last Update: 2020-03-30