[1]伍晓红 王启松 骆 凯 陈世炜.骨膜源性细胞和骨髓间充质干细胞直接共培养的实验研究[J].福建医药杂志,2019,41(04):118-121.
 WU Xiaohong,WANG Qisong,LUO Kai,et al.Experimental study on direct co-culture of periosteum-derived cells and bone marrow mesenchymal stem cells[J].FUJIAN MEDICAL JOURNAL,2019,41(04):118-121.
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骨膜源性细胞和骨髓间充质干细胞直接共培养的实验研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
41
期数:
2019年04期
页码:
118-121
栏目:
基础研究
出版日期:
2019-08-15

文章信息/Info

Title:
Experimental study on direct co-culture of periosteum-derived cells and bone marrow mesenchymal stem cells
文章编号:
1002-2600(2019)04-0118-04
作者:
伍晓红 王启松1 骆 凯 陈世 炜2
福建医科大学附属口腔医院牙周科(福州 350002)
Author(s):
WU Xiaohong WANG Qisong LUO Kai CHEN Shiwei
Department of Periodontology, the Affiliated Stomatological Hospital,Fujian Medical University,Fuzhou, Fujian 350002,China
关键词:
骨膜细胞 骨髓基质细胞 共培养 成骨分化 组织工程
Keywords:
periosteum bone marrow mesenchymal stem cells coculture techniques tissue engineering
分类号:
R336
文献标志码:
B
摘要:
目的 探讨骨膜源性细胞(PDCs)和骨髓间充质干细胞(BMSCs)不同比例下直接共培 养对细胞的增殖及成骨分化的影响,优选细胞直接共培养的适宜比例。方法 取PDCs和BMSCs, 在保证每组总细胞数一致的情况下,分别以PDCs和 BMSCs按2:1、1:1、1:2、1:0、0:1比例混 合培养,通过细胞增殖能力,碱性磷酸酶活性(alkaline phosphatase activity, ALP)和矿化 结节形成的测定,来观察PDCs和BMSCs不同比例共培养下细胞增殖和成骨分化能力。结果 PDCs和BMSCs直接共培养(2:1、1:1、1:2)的细胞增殖能力高于单独PDCs组(1:0组)和单独 BMSCs组(0:1组),但差异无统计学意义(P>0.05),共培养组细胞ALP表达较对照组 高(P<0.05),2:1组ALP表达最高(P<0.05),同时共培养组矿化结节形成 较多,1:0组和0:1组矿化结节形成较疏散。结论 PDCs和BMSCs直接共培养有利于细胞的增殖 能力和成骨能力,以2:1比例直接共培养时最佳,适宜进一步的组织工程研究。
Abstract:
Objective To investigate the effects of direct co-culture ratio on the proliferation and osteogenic differentiation of PDCs and BMSCs, and to optimize the co-culture ratio.Methods PDCs and BMSCs were cultured at the ratio of 2:1, 1:1,1:2,1:0,0:1 respectively, and the total number of cells in each group was the same.The proliferation and osteogenic differentiation of PDCs and BMSCs co-cultured were observed by cell proliferation, alkaline phosphatase activity(ALP), and mineralized nodules formation.Results The cell proliferation of the co-culture group were higher than that of 1:0 and 0:1 groups, but there was no statistical differences between all groups(P>0.05).The ALP expression level of the co-culture group(2:1,1:1 and 1:2 ratio)especially in 2:1 ratio expressed higher than that of control groups(1:0 and 0:1 ratio) (P<0.05), and the mineralized nodules in co-culture groups were formed more than that in the control groups(1:0 and 0:1 ratio).Conclusion When PDCs and BMSCs was directly co-cultured at 2:1 ratio, the total cell proliferation and osteogenic ability are stronger than other groups, which are suitable for further tissue engineering research.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:2015年福建省中青年教师教育科研项目(JA15199) 1 福建医科大学口腔医学重点实验室; 2 通信作者
更新日期/Last Update: 2019-08-15