[1]衣 欢 林丹玫 郑祥钦 吴花敏 林 蓉 庄炜民 杨丽婷.邻苯二甲酸酯暴露与子宫内膜异位症疼痛关系的研究[J].福建医药杂志,2019,41(03):5-8.
　YI Huan,LIN Danmei,ZHENG Xiangqin,et al.Study of association between exposure to phthalates and endometriosis-related pain[J].FUJIAN MEDICAL JOURNAL,2019,41(03):5-8.
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邻苯二甲酸酯暴露与子宫内膜异位症疼痛关系的研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
41
期数:
2019年03期
页码:
5-8
栏目:
临床研究
出版日期:
2019-02-20

文章信息/Info

Title:
Study of association between exposure to phthalates and endometriosis-related pain
文章编号:
1002-2600(2019)03-0005-04
作者:
衣 欢 林丹玫1 郑 祥钦 吴花敏 林 蓉 庄炜民 杨丽婷
福建医科大学附属医院 福建省妇幼保健院妇产科(福州 350001)
Author(s):
YI Huan; LIN Danmei;  ZHENG Xiangqin;  WU Huamin; LIN Rong; ZHUANG Weimin; YANG Liting.
Fujian Provincial Maternal and Children Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou,Fujian 350007,China
关键词:
子宫内膜异位症;  邻苯二甲酸酯;  疼痛;  补体;  凝固系统;  基因芯片
Keywords:
endometriosis;  phthalates;  pain;  complement;  coagulation system;  gene chip
分类号:
R711.71
文献标志码:
A
摘要:
目的 研究环境内分泌干扰物对子宫内膜异位症疼痛的影响,探讨相关发病机制。方 法 采用气相色谱质谱联用技术质谱法检测子宫内膜异位症患者和正常对照者尿液中邻苯二甲酸 酯(PAEs)9种代谢物含量,量化分析子宫内膜异位症患者痛经情况,研究代谢物和痛经的相关性。 通过合并痛经子宫内膜异位症患者基因芯片筛选疼痛相关通路和关键蛋白(GSE23339)。结果 子 宫内膜异位症患者尿液中邻苯二甲酸-单-乙基己基酯(MEHP)、邻苯二甲酸单甲酯(MMP)含量均明 显高于正常对照(5.67±2.01)μg /L vs(2.54±0.85)μg /L和 (17.11±8.37)μg /L vs(6.67±2.01)μg /L,均P<0.001。MEHP与 患者痛经VAS评分呈正相关(r=0.86,P<0.001)。基因测序分析表明子宫内膜 异位症并发疼痛患者体内补体和凝固级联通路明显激活,重要调节蛋白有人补体成分1S(C1S)、 补体成分7(C7)、补体因子H(CFH)和蛋白S1(PROS1)。结论 PAEs与子宫内膜异位症发病有关,可 能通过补体和凝固级联通路介导子宫内膜异位症相关疼痛。
Abstract:
Objective To study the effects of endocrine disruptors on endometriosis-related pain and explore the pathogenesis of endometriosis-related pain.Methods Nine metabolites of phthalic acid esters(PAEs)in urine of patients with endometriosis and normal controls were detected by mass spectrometry.The dysmenorrhea of endometriosis patients was quantitatively analyzed, and the correlation between metabolites and dysmenorrhea was studied.The pain-related pathways and key proteins were screened by gene microarray in endometriosis who complicated with dysmenorrhea(GSE23339).Results The concentration of MEHP and MMP in the urine of endometriosis were significantly higher than those of the normal control [(5.67±2.01)μg/L vs(2.54±0.85)μg/L and(17.11±8.37) μg/L vs(6.67±2.01)μg/L,(P<0.001, respectively)].MEHP was positively correlated with VAS score of dysmenorrhea.Gene sequencing analysis showed that complement and coagulation cascades were activated in patients with endometriosis complicated with pain, and the important regulatory proteins were C1S, C7, CFH and PROS1.Conclusion PAEs are associated with the pathogenesis of endometriosis and may mediate endometriosis-related pain through complement and coagulation cascade pathways.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金项目(2016J01429) 1 通信作者,福建省福州市妇幼保健院(福州 350005), Email:1013730089@qq.com
更新日期/Last Update: 2019-02-20