[1]林昌建.尼可地尔对TGF-β1诱导人气道平滑肌细胞肥大的实验研究[J].福建医药杂志,2019,41(01):109-112.
　LIN Changjian..Effects of Nicoorandil on TGF-β1-induced human airway smooth muscle cells hypertrophy[J].FUJIAN MEDICAL JOURNAL,2019,41(01):109-112.
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尼可地尔对TGF-β1诱导人气道平滑肌细胞肥大的实验研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
41
期数:
2019年01期
页码:
109-112
栏目:
基础研究
出版日期:
2019-01-20

文章信息/Info

Title:
Effects of Nicoorandil on TGF-β1-induced human airway smooth muscle cells hypertrophy
文章编号:
1002-2600(2019)01-0109-04
作者:
林昌建
福建省立医院呼吸与危重症医学科(福州 350001)
Author(s):
LIN Changjian.
Department of Pulmonary and Critical Care Medicine, Fujian Provincial Hospital,Fuzhou,Fujian 350001,China
关键词:
KATP;  TGF-β1;  气道平滑肌细胞;  肥大;  α-SMA;  CaMKⅡ
Keywords:
KATP;  TGF-β1;  ASMCs;  hypertrophy;  α-SMA;  CaMKⅡ
分类号:
R562.2+5
文献标志码:
A
摘要:
目的 研究ATP敏感性钾通道(KATP)开放剂对人气道平滑肌细胞(HASMCs)肥大的影响及其可能的作用机制。方法 用TGF-β1诱导HASMCs肥大,ATP敏感性钾通道开放剂尼可地尔(Nico)进行干预,将实验分成Control组、TGF-β1组、TGF-β1+Nico组、Nico组。显微镜下及免疫荧光法观察细胞形态变化及α-SMA表达情况; Western Blot法测定细胞α-SMA蛋白表达和CaMKⅡ、CREB磷酸化情况。结果 免疫荧光实验表明TGF-β1能浓度依赖性地促进细胞肥大和α-SMA表达,Nico能下调α-SMA的表达,减轻细胞肥大; Western blot实验表明TGF-β1能升高α-SMA表达和CaMKⅡ、CREB磷酸化水平,Nico能抑制α-SMA的表达,降低CaMKⅡ、CREB磷酸化水平。结论 Nico能减轻 TGF-β1诱导的HASMCs肥大,可能与抑制CaMKⅡ/CREB信号通路传导有关。
Abstract:
Objective To investigate the effects of the ATP sensitive potassium(KATP)channel openers on human airway smooth muscle cells(HASMCs)hypertrophy and the mechanism underlying. Methods The hypertrophy of HASMCs was induced by exposing to TGF-β1 in vitro.With the intervention of KATP channel opener(Nicorandil),the experiment was divided into four groups:control group, TGF-β1 group, TGF-β1+Nico group and Nico group.The cellular morphological changes and the expression of α-SMA were observed by the microscope and immunofluorescence,the protein expression of α-SMA and phosphorylation of CaMKⅡ,CREB were detected by Western blot.Results Nicorandil could suppress TGF-β1-induced HASMCs hypertrophy by reducing the expression of α-SMA and phosphorylation of CaMKⅡ,CREB.Conclusion Nicorandil can inhibit TGF-β1-induced HASMCs hypertrophy by reducing the phosphorylation of CaMKⅡ/CREB signaling pathways.

参考文献/References:

[1] Goldsmith A M, Bentley J K, Zhou L,et al. Transforming growth factor-beta induces airway smooth muscle hypertrophy[J]. Am J Respir Cell Mol Biol, 2006,34(2): 247-254.
[2] Wan X,Zhao J,Xie J.Effects of mitochondrial ATP-sensitive K(+)channel on protein kinase C pathway and airway smooth muscle cell proliferation in asthma[J].J Huazhong Univ Sci Technolog Med Sci,2012,32(4):480-484.
[3] Heinz F, Christina W, Michael W. Airway remodeling in asthma: what really matters [J]. Cell Tissue Res,2017, 367(3): 551-569.
[4] 朱斌,赵金宪,叶铁虎.ATP敏感性钾通道的阻断剂与开放剂研究进展[J].中国临床药理学杂志,2005,2(1):70-73.
[5] Amy M S, Yongfeng L, Li-Yan S,et al. Smooth Muscle CaMKIIδ Promotes Allergen Induced Airway Hyperresponsiveness and Inflammation[J]. Pflugers Arch,2015,467(12): 2541-2554.
[6] Jaggar J H,Porter V A,Lederer W J,et al.Calcium sparks in smooth muscle[J].Am J Physiol Cell Physiol,2000,278(2):C235-256.
[7] Yokoshiki H,Sunagawa M,Seki T,et al.ATP-sensitive K+ channels in pancreatic,cardiac and vascular smooth cells[J].Am J Physiol,1998,274(1 Pt1):C25-37.
[8] Jorge R, Edgar F, Abril C, et al. Maintenance of intracellular Ca2+ basal concentration in airway smooth muscle [J]. Int J Mol Med,2018,42(6): 2998-3008.
[9] Liu W, Kong H, Zeng X, et al. Iptakalim inhibits PDGF-BB-induced human airway smooth muscle cells proliferation and migration[J]. Exp Cell Res,2015,336(2):204-210.
[10] El-Kashef D H. Nicorandil alleviates ovalbumin-induced airway inflammation in a mouse model of asthma [J]. Environ Toxicol Pharmacol,2018,59:132-137.
[11] Christie A,Ojiaku E,Yoo P. Transforming growth factor β1 function in airway remodeling and hyperresponsiveness[J]. The Missing Link Am J Respir Cell Mol Biol, 2017,56(4): 432-442.
[12] 石永英,陈广原,刘世明,等.TGF-β1诱导大鼠心肌细胞肥大中钙调素依赖蛋白激酶Ⅱ信号途径研究[J].广州医学院学报,2011(3):21-25.
[13] Huan M, Rachel D, Groth S,et al. γCaMKII shuttles Ca2+/CaM to the nucleus to trigger CREB phosphorylation and gene expression[J]. Cell, 2014,159(2): 281-294.
[14] Zhou B, Liu Y, Kahn M,et al. Interactions between β-catenin and transforming growth factor-β signaling pathways mediate epithelial-mesenchymal transition and are dependent on the transcriptional co-activator cAMP-response element-binding protein(CREB)-binding protein(CBP)[J]. J Biol Chem,2012,287(10):7026-7038.

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更新日期/Last Update: 2019-01-20