[1]李渊根 郑兴中 黄荣桂.熊果酸对阿霉素肾病小鼠肾脏病变的影响[J].福建医药杂志,2018,40(06):123-125.
　LI Yuangen,ZHENG Xingzhong,HUANG Ronggui..Intervention effect of ursolic acid on adriamycin-induced nephropathy in mice[J].FUJIAN MEDICAL JOURNAL,2018,40(06):123-125.
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熊果酸对阿霉素肾病小鼠肾脏病变的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
40
期数:
2018年06期
页码:
123-125
栏目:
基础研究
出版日期:
2018-11-29

文章信息/Info

Title:
Intervention effect of ursolic acid on adriamycin-induced nephropathy in mice
文章编号:
1002-2600(2018)06-0123-04
作者:
李渊根 郑兴中1 黄荣桂2
福建医科大学附属第二医院肾脏内科(泉州 362000)
Author(s):
LI Yuangen;  ZHENG Xingzhong;  HUANG Ronggui.
Department of Nephrology,the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China
关键词:
熊果酸;  阿霉素肾病;  TGF-β1;  肾小管间质纤维化
Keywords:
ursolic acid;  adriamycin-induced nephropathy;  TGF-β1;  renal tubulointerstitial fibrosis
分类号:
R96
文献标志码:
B
摘要:
目的 研究熊果酸(ursolic acid,UA)对小鼠阿霉素肾病(adriamycin-induced nephropathy,ADN)的影响,探讨其可能的机制。方法 40只Balb/c小鼠,随机分为正常对照组(Normal组),阿霉素肾病组(ADN组),低剂量熊果酸组(LU+ADN组),高剂量熊果酸组(HU+ADN组)。单次注射阿霉素创建阿霉素肾病模型,以熊果酸为干预因素,测定尿蛋白总量,苏木素-伊红(HE)染色观察肾脏病变情况,免疫组织化学法检测TGF-β1在肾小管间质中的表达。结果 1)小鼠24 h尿蛋白总量:ADN组小鼠较Normal组显著升高(P<0.05),HU+ADN组较ADN组显著下降(P<0.05)。2)HE染色:ADN组出现显著的肾小管上皮细胞肿胀,管腔扩张等病变。与ADN组相比,HU+ADN组肾小管间质病变较轻。3)免疫组化染色半定量分析:①与Normal组比,ADN组肾间质表达TGF-β1显著增加,差异有统计学意义(P<0.05)。②与ADN组比,LU+ADN组TGF-β1表达差异无统计学意义(P>0.05); HU+ADN组小鼠TGF-β1表达显著下降,两组差异具有统计学意义(P<0.05)。结论 高剂量熊果酸(50 mg/kg)可以减少阿霉素肾病小鼠的尿蛋白,抑制阿霉素肾病小鼠肾小管间质TGF-β1蛋白的表达,发挥肾脏保护作用。
Abstract:
Objective To investigate the effect and mechanism of ursolic acid on adriamycin-induced nephropathy in mice. Methods Forty Balb/c mice were randomly divided into control group(Normal group),ADN group,low dose of UA group(LU+ADN group),high dose of UA group(HU+ADN group). The ADN model was established by treating with a single tail vein injection of ADR, UA was regarded as the interference factor. The Normal group received a single tail vein injection of saline only. Twenty four-hour urine was collected by urine protein tests. HE staining was used to obverse the tubulointerstitial lesions, and immunohistochemical staining detected the expression of TGF-β1 in tubulointerstitium. Results 1)24-hour urinary protein excretion:At day 7 after ADR injection, albuminuria was most evident in ADN group as compared with Normal group(P<0.05), and was attenuated in HU+ADN group as compared with ADN group(P<0.05). 2)HE staining: The renal tubulointerstitial lesions of ADN group mice were obvious, with swelling of tubular epithelial cells, tubular dilation obviously. The tubulointerstitial lesions of HU+ADN group mice were relatively relieved. 3)The semi-quantitative analysis of immunohistochemical staining: Compared with Normal group, the expression level of TGF-β1 in renal tissue of ADN group was significantly increased(P<0.05). Compared with ADN group, difference of the expression level of TGF-β1 in renal tissue of LU+ADN group had no statistics significance(P>0.05). Compared with ADN group, the expression level of TGF-β1 in renal tissue of HU+ADN group was significantly reduced(P<0.05). Conclusion The high concentration of UA(50 mg/kg)has a protection effect on mice with adriamycin-induced nephropathy by reducing urinary protein excretion and inhibiting the TGF-β1 protein expression in tubulointerstitial.

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更新日期/Last Update: 2018-11-29