[1]梁杰,陈舜喜,卞智淮,等.超声波对兔膝关节骨关节炎关节滑膜和滑液VEGF及MMP-13表达的影响[J].福建医药杂志,2025,47(03):61-66.[doi:10.20148/j.fmj.2025.03.016]
　LIANG Jie,CHEN Shunxi,BIAN Zhihuai,et al.Influence of ultrasound on expression of VEGF and MMP-13 in joint synovium and synovial fluid of rabbits with knee osteoarthritis[J].FUJIAN MEDICAL JOURNAL,2025,47(03):61-66.[doi:10.20148/j.fmj.2025.03.016]
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超声波对兔膝关节骨关节炎关节滑膜和滑液VEGF及MMP-13表达的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
47
期数:
2025年03期
页码:
61-66
栏目:
基础研究
出版日期:
2025-03-20

文章信息/Info

Title:
Influence of ultrasound on expression of VEGF and MMP-13 in joint synovium and synovial fluid of rabbits with knee osteoarthritis
文章编号:
1002-2600(2025)03-0061-06
作者:
梁杰1; 陈舜喜1; 卞智淮1; 朱雨珍2; 郑明煜2; 陈小莉2
1 福建省福州市第二总医院康复医学科,福州 350007; 2 福建中医药大学,福州 350013
Author(s):
LIANG Jie1;  CHEN Shunxi1;  BIAN Zhihuai1;  ZHU Yuzhen2;  ZHENG Mingyu2;  CHEN Xiaoli2
1 Department of Rehabilitation Medicine, The Second General Hospital of Fuzhou,Fuzhou, Fujian 350007, China; 2 Fujian University of Chinese Medicine, Fuzhou, Fujian 350013, China
关键词:
超声波;  大白兔;  膝关节;  基质金属蛋白酶-13;  血管内皮生长因子
Keywords:
ultrasound;  rabbit;  knee joint;  matrix metalloproteinase-13;  vascular endothelial growth factor
分类号:
R49
DOI:
10.20148/j.fmj.2025.03.016
文献标志码:
A
摘要:
目的 探讨超声波对兔膝关节骨关节炎(knee osteoarthritis,KOA)关节滑膜和关节滑液血管内皮生长因子(vascular endothelial growth factor,VEGF)、基质金属蛋白酶-13(matrix metalloproteinases-13, MMP-13)表达的影响。方法 选取24只新西兰大白兔随机分为假手术组(sham-operated group,SO组)、骨关节炎组(osteoarthritis group,OA组)和超声治疗组(ultrasound treatment group,UT组)。采用前交叉韧带横断术(anterior cruciate ligament transection,ACLT)法造模,UT组予超声波治疗仪治疗兔膝关节; OA组处理同上,但未打开超声波治疗仪; SO组未做处理。干预2周后,抽取关节液标本,并观察标本滑膜、软骨的大体形态; 酶联免疫吸附法检测关节滑液VEGF和MMP-13的表达; Western blot方法检测关节滑膜VEGF和MMP-13的表达。结果 与SO组相比,OA组和UT组兔膝关节骨关节炎评分均显著升高(P<0.05); 与OA组相比,UT组兔膝关节骨关节炎评分显著降低(P<0.05)。与SO组相比,OA组和UT组兔膝关节滑液及滑膜中VEGF和MMP-13的蛋白水平均显著升高(P<0.05); 与OA组相比,UT组兔膝关节滑液及滑膜中VEGF和MMP-13的蛋白水平显著降低(P<0.05)。结论 超声波治疗能明显抑制兔膝关节滑膜增生,有效抑制关节滑液和滑膜组织中VEGF、MMP-13的表达水平,从而达到防治KOA的作用。
Abstract:
Objective To investigate the influence of ultrasound on the expression of VEGF and MMP-13 in the joint synovium and synovial fluid of rabbit with knee osteoarthritis(KOA). Methods Twenty-four New Zealand white rabbits were randomly assigned into three groups: the sham-operated group(SO group), the osteoarthritis group(OA group), and the ultrasound treatment group(UT group).The model was made by anterior cruciate ligament transection. The UT group was treated with an ultrasound therapy device on the rabbit knee joint; the OA group received the same treatment but the ultrasound therapy device was not activated; the SO group received no treatment. After two weeks of intervention, joint fluid specimens were extracted, and the gross morphology of the synovial membrane and cartilage of the specimens was observed. The expression of VEGF and MMP-13 in the joint fluid was detected by enzyme-linked immunosorbent assay(ELISA), and the expression of VEGF and MMP-13 in the joint synovial membrane was detected by Western blot method. Results Compared with the SO group, the OA and UT groups showed significantly increased osteoarthritis markers in the rabbit knee joints(P<0.05); compared with the OA group, the UT group showed significantly decreased osteoarthritis markers in the rabbit knee joints(P<0.05). Compared with the SO group, the OA and UT groups showed significantly increased VEGF and MMP-13 protein levels in the rabbit knee joint synovial fluid and synovium(P<0.05); compared with the OA group, the UT group showed significantly decreased VEGF and MMP-13 protein levels in the rabbit knee joint synovial fluid and synovium(P<0.05). Conclusion Ultrasound therapy can significantly inhibit the proliferation of synovial membrane in rabbit knee joints, slow down the progression of osteoarthritis, effectively reduce the expression levels of VEGF and MMP-13 in synovial fluid and synovial tissue, and thus achieve the goal of preventing and treating KOA.

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金项目(2023J011508); 福建省科技创新平台项目(福建省创伤骨科急救与康复临床医学研究中心,2020Y2014)
通信作者:陈舜喜,Email:csx123@sina.com
更新日期/Last Update: 2025-03-20