[1]梁玲,方豆豆.肿瘤微环境中黑磷纳米片对腺苷激酶的调控研究[J].福建医药杂志,2025,47(02):51-55.[doi:10.20148/j.fmj.2025.02.014]
　LIANG Ling,FANG Doudou.Regulation of black phosphorus nanosheets on adenosine kinase in tumor microenvironment[J].FUJIAN MEDICAL JOURNAL,2025,47(02):51-55.[doi:10.20148/j.fmj.2025.02.014]
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肿瘤微环境中黑磷纳米片对腺苷激酶的调控研究()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
47
期数:
2025年02期
页码:
51-55
栏目:
基础研究
出版日期:
2025-02-20

文章信息/Info

Title:
Regulation of black phosphorus nanosheets on adenosine kinase in tumor microenvironment
文章编号:
1002-2600(2025)02-0051-05
作者:
梁玲; 方豆豆
闽江学院材料与化学工程学院,福州 350108
Author(s):
LIANG Ling;  FANG Doudou
College of Materials and Chemical Engineering,Minjiang University,Fuzhou,Fujian 350108,China
关键词:
黑磷;  肿瘤微环境;  腺苷激酶;  活性调控
Keywords:
black phosphorus;  tumor microenvironment;  adenosine kinase;  activity regulation
分类号:
O643; Q555
DOI:
10.20148/j.fmj.2025.02.014
文献标志码:
B
摘要:
目的 基于磷酸盐(PO43-)调控的腺苷激酶(ADK)活性,探究黑磷纳米片(BPNSs)的选择性降解对ADK活性的调控。方法 利用中性和酸性两种溶液分别模拟正常生理环境、肿瘤微环境,通过吸收强度及PO43-含量测定探究BPNSs在两种溶液中的降解特性; 并通过酶促反应动力学探究BPNSs在两种溶液中对ADK催化腺苷磷酸化反应的调控。结果 BPNSs在模拟的肿瘤微环境中更易降解,产生的PO43-明显高于模拟的正常生理环境。ADK在两种溶液中均能催化腺苷磷酸化,然而其活性受高浓度腺苷抑制。受益于在模拟的肿瘤微环境中降解产生高含量的PO43-,BPNSs恢复了ADK活性并促进了ADK催化的腺苷转化。结论 基于黑磷纳米材料降解特性和酶活性调控机制,BPNSs有望成为新型腺苷代谢调控药物和腺苷介导的免疫抑制调节剂。
Abstract:
Objective To investigate the selective degradation of black phosphorus nanosheets(BPNSs)on the regulation of adenosine kinase(ADK)activity regulated by phosphate(PO43-).Methods Neutral and acidic solutions were used to simulate normal physiological environment and tumor microenvironment, respectively.The degradation of BPNSs in both solutions were explored by measuring absorption intensity and PO43-.The regulation of BPNSs on ADK catalyzed adenosine phosphorylation in both solutions was investigated through the kinetic of enzyme-catalyzed reaction.Results In the simulated tumor microenvironment, BPNSs were degraded more easily and produced higher concentrations of PO43-.ADK could catalyze adenosine phosphorylation in both solutions, but its activity was affected by high concentration of adenosine.Benefiting from the degradation to produce high level of PO43-, BPNSs restored ADK activity and promoted ADK-catalyzed adenosine transformation in the simulated tumor microenvironment.Conclusion Based on the degradation of black phosphorus and the regulation mechanism of enzyme activity, BPNSs may become novel adenosine metabolism regulating drugs and adenosine-mediated immunosuppressive regulators.

参考文献/References:

[1] Hui L,Chen Y.Tumor microenvironment: Sanctuary of the devil[J].Cancer Lett,2015,368(1):7-13.
[2] Chen J F,Eltzschig H K,Fredholm B B.Adenosine receptors as drug targets— what are the challenges?[J].Nat Rev Drug Discovery,2013,12(4):265-286.
[3] Vijayan D,Young A,Teng M W L,et al.Targeting immunosuppressive adenosine in cancer[J].Nat Rev Cancer,2017,17(12):709-724.
[4] Boison D,Yegutkin G G.Adenosine metabolism: Emerging concepts for cancer therapy[J].Cancer Cell,2019,36(6):582-596.
[5] Arab S,Hadjati J.Adenosine Blockage in tumor microenvironment and improvement of cancer immunotherapy[J].Immune Netw,2019,19(4):e23.
[6] Boison D.Adenosine kinase:exploitation for therapeutic gain[J].Pharmacol Rev,2013,65(3):906-943.
[7] Xu Y,Wang Y,Yan S,et al.Regulation of endothelial intracellular adenosine via adenosine kinase epigenetically modulates vascular inflammation[J].Nat Commun,2017,8(1):943.
[8] Maj M,Singh B,Gupta R S.The influence of inorganic phosphate on the activity of adenosine kinase[J].Biochim Biophys Acta,2000,1476(1):33-42.
[9] Park J,Singh B,Maj M C,et al.Phosphorylated derivatives that activate or inhibit mammalian adenosine kinase provide insights into the role of pentavalent ions in AK catalysis[J].Protein J,2004,23(2):167-177.
[10] Zhao C,Chen J,Zhong R,et al.Oxidative-species-selective materials for diagnostic and therapeutic applications[J].Angew Chem Int Ed,2021,60(18):9804-9827.
[11] Guo Z,Zhang H,Lu S,et al.From black phosphorus to phosphorene: Basic solvent exfoliation,evolution of raman scattering,and applications to ultrafast photonics[J].Adv Funct Mater,2015,25(45):6996-7002.
[12] Beavis P A,Divisekera U,Paget C,et al.Blockade of A2A receptors potently suppresses the metastasis of CD73+ tumors[J].Proc Natl Acad Sci,2013,110(36):14711.
[13] Stagg J,Divisekera U,Mclaughlin N,et al.Anti-CD73 antibody therapy inhibits breast tumor growth and metastasis[J].Proc Natl Acad Sci,2010,107(4):1547.
[14] Zhao W,Xue Z,Wang J,et al.Large-scale,highly efficient,and green liquid-exfoliation of black phosphorus in ionic liquids[J].ACS Appl Mater Interfaces,2015,7(50):27608-27612.
[15] Zhang S,Zhang X,Lei L,et al.pH-dependent degradation of layered black phosphorus: Essential role of hydroxide ions[J].Angew Chem Int Ed,2019,58(2):467-471.
[16] Zhou W,Pan T,Cui H,et al.Black phosphorus:bioactive nanomaterials with inherent and selective chemotherapeutic effects[J].Angew Chem Int Ed,2019,58(3):769-774.
[17] Spychala J.Tumor-promoting functions of adenosine[J].Pharmacol Ther,2000,87(2-3):161-173.
[18] He Z,Chen W,Hu K,et al.Resolvin D1 delivery to lesional macrophages using antioxidative blackphosphorus nanosheets for atherosclerosis treatment[J].Nat Nanotechnol,2024,19(9):1386-1398.

备注/Memo

备注/Memo:
基金项目:福建省大学生创新创业训练计划项目(S202210395055); 福建省中青年教师教育科研项目(JAT220328); 福建省自然科学基金项目(2023J05245)
更新日期/Last Update: 2025-02-20