[1]谢颖,郑志海,曲双,等.外周血宏基因组二代测序技术对恶性血液病合并发热患者的诊断价值[J].福建医药杂志,2024,46(07):12-15.[doi:10.20148/j.fmj.2024.07.004]
　XIE Ying,ZHENG Zhihai,QU Shuang,et al.Diagnostic value of peripheral blood metagenomic next-generation sequencing technology in patients with malignant hematologic diseases complicated with fever[J].FUJIAN MEDICAL JOURNAL,2024,46(07):12-15.[doi:10.20148/j.fmj.2024.07.004]
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外周血宏基因组二代测序技术对恶性血液病合并发热患者的诊断价值()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年07期
页码:
12-15
栏目:
临床研究
出版日期:
2024-11-20

文章信息/Info

Title:
Diagnostic value of peripheral blood metagenomic next-generation sequencing technology in patients with malignant hematologic diseases complicated with fever
文章编号:
1002-2600(2024)07-0012-04
作者:
谢颖; 郑志海; 曲双; 廖丽昇; 陈碧云
福建医科大学省立临床医学院 福建省立医院血液科 福州大学附属省立医院,福州 350001
Author(s):
XIE Ying;  ZHENG Zhihai;  QU Shuang;  LIAO Lisheng;  CHEN Biyun
Department of Hematology,Fujian Provincial Hospital, Shengli Clinical College of Fujian Medical University, Fuzhou University Affiliated Provincial Hospital, Fuzhou, Fujian 350001, China
关键词:
恶性血液病;  外周血宏基因组二代测序技术;  发热
Keywords:
malignant hematologic diseases;  peripheral blood metagenomic next-generation sequencing technology;  fever
分类号:
R552
DOI:
10.20148/j.fmj.2024.07.004
文献标志码:
B
摘要:
目的 探讨外周血宏基因组二代测序(mNGS)技术对恶性血液病合并发热患者的诊断价值。方法 回顾性分析2021年6月至2023年6月在我院住院治疗的恶性血液病合并发热的患者23份外周血mNGS结果,与传统血培养检测结果进行比较,分析其病原谱特征及抗感染的疗效。结果 23例患者中,通过外周血mNGS技术检出9例病毒感染、4例细菌感染、2例真菌感染、3例病毒和细菌混合型感染、1例病毒和真菌混合型感染。传统外周血培养仅检出3例细菌感染,主要为肺炎克雷伯菌及鲍曼不动杆菌,未检出真菌及病毒。外周血mNGS阳性检出率明显高于传统血培养(82.6% vs.13.0%, P<0.05)。根据外周血mNGS检测结果进行针对性抗感染治疗,有效率明显优于传统经验性抗感染治疗(73.6% vs.25.0%, P<0.05)。结论 外周血mNGS技术在恶性血液病合并发热患者的病原学诊断上较传统血培养具有更高的价值,有助于提高临床抗感染的疗效。
Abstract:
Objective To investigate the diagnostic value of peripheral blood metagenomic next-generation sequencing(mNGS)in patients with malignant hematologic diseases complicated with fever.Methods Twenty-three peripheral blood mNGS results from patients with malignant hematologic diseases complicated with fever who were hospitalized in Fujian Provincial Hospital from June 2021 to June 2023 were restrospectively analyzed, and compared with traditional blood culture tests.The characteristics of the pathogenic spectrum and the efficacy of anti-infection were analyzed.Results Among the 23 patients detected by peripheral blood mNGS technology, 9 cases were diagnosed of viral infection, 4 cases of bacterial infection, 2 cases of fungal infection, 3 cases of mixed viral and bacterial infection, and 1 case of mixed viral and fungal infection.Only three cases detected in traditional peripheral blood culture were diagnosed of bacterial infection, mainly Klebsiella pneumoniae and Acinetobacter baumannii, but no fungi and viruses were detected.The positive detection rate of mNGS in peripheral blood was significantly higher than that in traditional blood culture(82.6% vs.13.0%, P<0.05).The effective rate of targeted anti-infective therapy based on the results of peripheral blood mNGS was significantly higher than that of traditional empirical anti-infective therapy(73.6% vs.25.0%, P<0.05). Conclusion Compared with traditional blood culture, peripheral blood mNGS technology has higher positive detection rate than traditional blood culture in the etiological diagnosis of patients with malignant hematologic diseases complicated with fever, which is helpful to improve the clinical anti-infection efficacy.

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备注/Memo

备注/Memo:
通信作者:陈碧云,Email:253934019@qq.com
更新日期/Last Update: 2024-11-20