[1]谢文焌,李华水,林强.miR-149-3p通过调控NIS促进甲状腺乳头状癌增殖和迁移[J].福建医药杂志,2024,46(04):94-99.[doi:10.20148/j.fmj.2024.04.028]
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miR-149-3p通过调控NIS促进甲状腺乳头状癌增殖和迁移()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年04期
页码:
94-99
栏目:
基础研究
出版日期:
2024-08-15

文章信息/Info

文章编号:
1002-2600(2024)04-0094-06
作者:
谢文焌李华水林强
福建省立医院甲状腺疝外科,福州 350001
关键词:
甲状腺肿瘤 miR-149-3p NIS 增殖活性 迁移
分类号:
R736.1
DOI:
10.20148/j.fmj.2024.04.028
文献标志码:
B
摘要:
目的 探讨miR-149通过调控钠/碘共同转运体(Sodium/Iodide Sympoter,NIS)调控甲状腺乳头状癌(Papillary Thyroid Carcinoma,PTC)细胞增殖和迁移能力。方法 对选取的GSE113629转录组数据进行数据处理,筛选其中表达差异较为明显且在PTC组织中表达明显增高的MicroRNA(miRNA)作为研究分子。通过在线数据库预测目标miRNA的下游靶基因,随后检测调控目标miRNA表达后靶基因变化情况并观察靶基因水平改变对甲状腺癌细胞生物学行为的影响。结果 在GSE112629数据集中,研究发现miR-149-3p在PTC组织中高表达。通过对miR-149-3p预测靶基因进行维恩分析,我们发现NIS是miR-149-3p的重要靶基因。通过分析GEPIA和UALCAN数据库,我们发现NIS在PTC样本中明显低表达,同时发现PTC的淋巴结转移率越高、分期级别越高则NIS表达量越低,并且NIS表达量与患者无病生存期(Disease-Free Survival,DFS)呈正相关。在多种PTC细胞系中miR-149-3p表达水平均高于正常甲状腺细胞系,抑制PTC细胞内miR-149-3p表达后细胞内NIS表达水平明显升高。当敲低miR-149-3p时,细胞的增殖活性和迁移能力受到明显抑制。结论 PTC中miR-149 -3p高表达,并且miR-149-3p 能够通过抑制NIS表达明显增强PTC的增殖活性和迁移能力。

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备注/Memo

备注/Memo:
基金项目:福建省卫生健康委青年科研基金(2020QNA007)
通信作者:李华水,Email: wuxingli83@163.com
更新日期/Last Update: 2024-08-15