[1]林滨,蔡沓栗,何小蓉,等.基于体外微量反应评价某些甾醇类化合物对5α还原酶的抑制作用[J].福建医药杂志,2024,46(04):91-94.[doi:10.20148/j.fmj.2024.04.027]
　LIN Bin,CAI Dali,HE Xiaorong,et al.Evaluation of the inhibitory effects of certain sterol compounds on 5 α-reductase based on in vitro micro reactions[J].FUJIAN MEDICAL JOURNAL,2024,46(04):91-94.[doi:10.20148/j.fmj.2024.04.027]
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基于体外微量反应评价某些甾醇类化合物对5α还原酶的抑制作用()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年04期
页码:
91-94
栏目:
基础研究
出版日期:
2024-08-15

文章信息/Info

Title:
Evaluation of the inhibitory effects of certain sterol compounds on 5 α-reductase based on in vitro micro reactions
文章编号:
1002-2600(2024)04-0091-04
作者:
林滨; 蔡沓栗; 何小蓉; 何宇; 江举
福州市皮肤病防治院,福州 350025
Author(s):
LIN Bin; CAI Dali; HE Xiaorong; HE Yu; JIANG Ju
Dermatology Hospital of FuZhou,Fuzhou,Fujian 350025,China
关键词:
5α还原酶;  非那雄胺;  体外;  脱发
Keywords:
5 α-reductase;  finasteride;  in vitro;  hair loss
分类号:
R914
DOI:
10.20148/j.fmj.2024.04.027
文献标志码:
B
摘要:
目的 基于体外微量反应,体外筛选有效的5α还原酶抑制物。方法 从SD大鼠肝脏中获得5α还原酶的粗酶,建立体外微量反应体系,通过反应前后睾酮量的变化来评价受试甾醇类化合物对5α还原酶的抑制作用。结果 在反应体系中加入二甲基亚砜(DMSO),解决非那雄胺及受试甾醇类化合物在水相中溶解度低等的问题,确保微量反应有效进行,从而得到可靠的筛选结果。结论 羊毛甾醇在体外具有较好的5α还原酶抑制效果,或许可以成为新型的5α还原酶抑制物。
Abstract:
Objective To screen effective inhibitors of 5 α-reductase in vitro,based on in vitro micro reactions. Methods The crude enzyme of 5 α-reductase was obtained from the liver of SD rats,and then established a micro reaction system in vitro. The inhibitory effect of the tested sterol compounds on 5 α-reductase was evaluated by the changes in testosterone levels before and after the reaction. Results The addition of dimethyl sulfoxide(DMSO)to the reaction system solved the problem of low solubility of finasteride and tested sterol compounds in the aqueous phase,ensuring effective trace reactions and obtaining reliable screening results. Conclusion Lanosterol has a good inhibitory effect on 5 α-reductase in vitro,and may become a new type of 5 α-reductase inhibitor.

参考文献/References:

[1] 郑瑞蓉,欧阳倩兮,刘子瑶.天然5α-还原酶抑制剂用于改善良性前列腺增生的研究进展[J].中国中药杂志,2024,49(4): 858-867.
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[3] 李慕春,张显文,王苗.12种植物的提取物对5α-还原酶的抑制活性研究[J].中南药学,2017,15(11): 1536-1540.
[4] LOFTSSON T,HREINSDóTTIR D.Determination of aqueous solubility by heating and equilibration: a technical note[J].AAPS Pharm Sci Tech,2006,7(1): 29-32.
[5] ZHANG R W,ZHANG X Q.Inhibitory activities of some traditional Chinese herbs against testosterone 5α-reductase and effects of cacumen platycladi on hair re-growth in testosterone-treated mice[J].J Ethnopharmacol,2016(177):1-9.

备注/Memo

备注/Memo:
基金项目:福州市科学技术局医疗卫生项目(2022-S-070); 福州市“十四五”临床专科培育建设项目整形外科(20220303)
通信作者:江 举,Email:846069532@qq.com
更新日期/Last Update: 2024-08-15