[1]汪家航,林明强,张捷平.NCAPG对大肠癌细胞HCT116增殖和凋亡的影响[J].福建医药杂志,2024,46(03):94-99.[doi:10.20148/j.fmj.2024.03.030]
　WANG Jiahang,LIN Mingqiang,ZHANG Jieping.Effects of NCAPG on the proliferation and apoptosis of colorectal cancer cell HCT116[J].FUJIAN MEDICAL JOURNAL,2024,46(03):94-99.[doi:10.20148/j.fmj.2024.03.030]
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NCAPG对大肠癌细胞HCT116增殖和凋亡的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年03期
页码:
94-99
栏目:
基础研究
出版日期:
2024-06-15

文章信息/Info

Title:
Effects of NCAPG on the proliferation and apoptosis of colorectal cancer cell HCT116
文章编号:
1002-2600(2024)03-0094-06
作者:
汪家航1; 林明强1; 张捷平2
1 福州市第二总医院胃肠外科,福州 350025; 2 福建中医药大学,福州 350122
Author(s):
WANG Jiahang1;  LIN Mingqiang1;  ZHANG Jieping2
1 Department of Gastrointestinal Surgery, Fuzhou Second General Hospital, Fuzhou, Fujian 350025, China; 2 Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350000, China
关键词:
非染色体结构维持蛋白复合体I G亚基;  大肠癌;  细胞生长;  凋亡
Keywords:
NCAPG;  colorectal cancer;  cell growth;  apoptosis
分类号:
R735.3+4
DOI:
10.20148/j.fmj.2024.03.030
文献标志码:
A
摘要:
目的 探讨非染色体结构维持蛋白复合体I G亚基(Non-SMC condensin I complex subunit G,NCAPG)对大肠癌细胞生长的作用和对增殖凋亡的影响。方法 敲减NCAPG的小干扰RNA(si-NCAPG)转染人大肠癌细胞株HCT116,Western blot检测si-NCAPG对大肠癌细胞中NCAPG蛋白表达的影响; 通过观察细胞形态、细胞活力评价si-NCAPG对大肠癌细胞生长的影响,并筛选效果最好的序列用于后续实验。通过克隆形成、细胞周期和凋亡、Western blot实验评价si-NCAPG对大肠癌细胞生长、周期和凋亡的影响。结果 si-NCAPG显著降低大肠癌细胞中NCAPG的蛋白表达(P<0.05),抑制大肠癌细胞数目和细胞活力(P<0.05),使细胞阻滞在G2/M期(P<0.05),并促进细胞凋亡(P<0.05)。敲减NCAPG降低Cyclin B1、CDK1和Bcl-2蛋白表达水平和升高BAX的蛋白表达水平(P<0.05)。结论 敲减NCAPG通过阻滞细胞在G2/M期和促进细胞凋亡,达到抑制大肠癌细胞生长的作用。
Abstract:
Objective To investigate the effect and mechanism of Non-SMC condensin I complex subunit G(NCAPG)on the growth,proliferation and apoptosis of colorectal cancer cells(CRC).Methods siRNA-NCAPG was transfected into human colorectal cancer cell line HCT116 cells.The effect of si-NCAPG on the expression of NCAPG in HCT116 cells was detected by Western blot.The effect of si-NCAPG on the growth of HCT116 cells was evaluated by observing cell morphology and cell viability, and the best sequences were screened for subsequent experiments.The effects of si-NCAPG on the cell growth, cycle and apoptosis of HCT116 cells were evaluated by clonal formation, cell cycle, apoptosis, and western blot.Results si-NCAPG significantly decreased the expression of NCAPG protein level(P<0.05), inhibited the cell number and cell viability(P<0.05), induced cell cycle arrest in G2/M phase(P<0.05)and promoted cell apoptosis in HCT116 cells(P<0.05).Knocking down NCAPG reduced expression levels of Cyclin B1, CDK1 and Bcl-2 protein and increased expression levels of BAX protein.Conclusion si-NCAPG significantly inhibits the growth of CRC by inducing cell cycle arrest at G2/M phase and promoting cell apoptosis.

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备注/Memo

备注/Memo:
基金项目:2021年福州市卫生健康中青年科学研究项目(2021-S-wq19); 福建省创伤骨科急救与康复临床医学研究中心项目(2020Y2014)
更新日期/Last Update: 2024-06-15