[1]郑述建,宋霖,严成,等.shRNA沉默circ_0089153对TPC-1甲状腺癌细胞增殖、迁移及侵袭的影响[J].福建医药杂志,2024,46(03):90-93.[doi:10.20148/j.fmj.2024.03.029]
　ZHENG Shujian,SONG Lin,YAN Cheng,et al.Effect of shRNA silencing circ_0089153 on proliferation, migration and invasion of TPC-1 thyroid cancer cells[J].FUJIAN MEDICAL JOURNAL,2024,46(03):90-93.[doi:10.20148/j.fmj.2024.03.029]
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shRNA沉默circ_0089153对TPC-1甲状腺癌细胞增殖、迁移及侵袭的影响()
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《福建医药杂志》[ISSN:1002-2600/CN:35-1071/R]

卷:
46
期数:
2024年03期
页码:
90-93
栏目:
基础研究
出版日期:
2024-06-15

文章信息/Info

Title:
Effect of shRNA silencing circ_0089153 on proliferation, migration and invasion of TPC-1 thyroid cancer cells
文章编号:
1002-2600(2024)03-0090-04
作者:
郑述建; 宋霖; 严成; 陈榕
福建省福州市第二总医院甲乳外科,福州350007
Author(s):
ZHENG Shujian;  SONG Lin;  YAN Cheng;  CHEN Rong
Department of Thyroid and Breast Surgery, Fuzhou Second General Hospital, Fuzhou, Fujian 350007, China
关键词:
甲状腺癌;  circ_0089153;  细胞增殖;  细胞迁移;  细胞侵袭
Keywords:
thyroid cancer;  circ_0089153;  cell proliferation;  cell migration;  cell invasion
分类号:
R73
DOI:
10.20148/j.fmj.2024.03.029
文献标志码:
B
摘要:
目的 探讨circ_0089153对TPC-1甲状腺癌细胞增殖、迁移及侵袭的影响。方法 采用circ_0089153 shRNA沉默TPC-1细胞中的circ_0089153表达并分成空白对照组、阴性对照组及shRNA沉默组,通过RT-PCR及Western blot检测不同组TPC-1细胞中circ_0089153 mRNA及蛋白的表达,采用CCK-8法检测不同组TPC-1细胞增殖,采用Transwell实验检测不同组细胞迁移和侵袭能力。结果 shRNA沉默组circ_0089153 mRNA及蛋白表达水平较空白对照组、阴性对照组低(P<0.05); 沉默hsa_circ_0089153后,TPC-1细胞增殖受到抑制; shRNA沉默组细胞增殖率、迁移和侵袭的细胞数较对照组低(P<0.05)。结论 circ_0089153与甲状腺癌细胞增殖、迁移及侵袭密切相关。
Abstract:
Objective To investigate the effects of circ_0089153 on the proliferation, migration and invasion of TPC-1 thyroid cancer cells.Methods The expression of circ_0089153 in TPC-1 cells was silenced by circ_0089153 shRNA and divi-ded into blank control group, negative control group and shRNA silenced group.The expression of circ_0089153 mRNA and protein in TPC-1 cells in different groups was detected by RT-PCR and Western blot, the proliferation of TPC-1 cells in different groups was detected by CCK-8 method, and the migration and invasion ability of TPC-1 cells in different groups was detected by transwell assay.Results The mRNA and protein expression levels of circ_0089153 in shRNA silencing group were lower than those in blank control group and negative control group(P<0.05).After silencing hsa_circ_0089153, TPC-1 cell proliferation was inhibited.The number of cell proliferation, migration and invasion in shRNA silencing group was lower than that in control group(P<0.05).Conclusion circ_0089153 is closely related to the proliferation, migration and invasion of thyroid cancer cells.

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金资助项目(2022J011322)
更新日期/Last Update: 2024-06-15